CAJ | 학술논문

以克隆到的果蔗HSP90基因片段作为探针,利用电子克隆和序列拼接方法获得一个全长为2 097 bp的cDNA序列.通过ORF软件分析.预测得到的蛋白质具有698个氨基酸.应用生物信息学对果蔗HSP90蛋白的理化性质、二级结构、疏水性,亲水性、信号肽、功能结构域分析及其蛋白功能、高级结构和同源性进行了预测分析.结果表明,该蛋白的相对分子量为80.2 ku,在N端有一个ATP结合位点,具有内源ATPase活性.序列分析表明,该蛋白可能具有信号转导、转录调控、胁迫应答、生长因子等功能.利用梢腐病病原Gibberella fujikuroi接种果蔗福安叶片,检测到HSP90基因的转录水平逐渐提高.
이극륭도적과자HSP90기인편단작위탐침,이용전자극륭화서렬병접방법획득일개전장위2 097 bp적cDNA서렬.통과ORF연건분석.예측득도적단백질구유698개안기산.응용생물신식학대과자HSP90단백적이화성질、이급결구、소수성,친수성、신호태、공능결구역분석급기단백공능、고급결구화동원성진행료예측분석.결과표명,해단백적상대분자량위80.2 ku,재N단유일개ATP결합위점,구유내원ATPase활성.서렬분석표명,해단백가능구유신호전도、전록조공、협박응답、생장인자등공능.이용소부병병원Gibberella fujikuroi접충과자복안협편,검측도HSP90기인적전록수평축점제고.
HSP90 cDNA fragment cloned from chewing cane(Saccharum officinarum L.) was used as probe to produce a 2097 bp cDNA fragment by using in silico cloning and splicing. The HSP90 cDNA sequence was predicted to have 698 amino acids by the ORF software. The HSP90 protein was analyzed by a series of bioinformatics software in terms of physical and chemical properties, second structure, hydrophobicity/ hydrophilicity, signal peptide, functional domain and protein function, high-level structure, and homology between different plants. The bioinformatics analysis showed that the HSP90 protein was 80.2 ku and contained a conserved ATPase domain with an ATP-binding site at the N end. Sequence analysis indicated that the HSP90 protein might function as signaling transduction, transcription regulation, stress response and a growing factor. The expression levels of HSP90 increased after the leaf of chewing cane was inoculated with Gibberella fujikuroi.