CAJ | 학술논문

采用改进的St(o)ber法制备了二氧化硅外壳的纳米复合荧光粒子.利用异硫氰酸荧光素(FITC)与3-氨基丙基三乙氧基硅烷(APTEOS)反应制备前驱体),再用正硅酸乙酯(TEOS)在一定的条件下水解与缩合,制备有机-无机纳米复合荧光颗粒,利用透射电子显微镜(TEM)测试表明,此纳米复合颗粒呈球形、大小均一,直径约为70 nm.制备的纳米复合荧光粒子经过多次水洗后,仍有较强的荧光特性,有效地防止FITC泄露.用激光共聚焦显微镜观测纳米复合荧光粒子标记的牛血清白蛋白(BSA),可以明显看出BSA上的绿色荧光.
채용개진적St(o)ber법제비료이양화규외각적납미복합형광입자.이용이류청산형광소(FITC)여3-안기병기삼을양기규완(APTEOS)반응제비전구체),재용정규산을지(TEOS)재일정적조건하수해여축합,제비유궤-무궤납미복합형광과립,이용투사전자현미경(TEM)측시표명,차납미복합과립정구형、대소균일,직경약위70 nm.제비적납미복합형광입자경과다차수세후,잉유교강적형광특성,유효지방지FITC설로.용격광공취초현미경관측납미복합형광입자표기적우혈청백단백(BSA),가이명현간출BSA상적록색형광.
Nano-composite fluorescence with silica shell had been prepared by improving St(o)ber route. The precursor was obtained via chemical reaction of fluorescein isothiocyanate(FITC) and 3-aminopropyltrietho-)xysilane(APTEOS)). Then, the controlled hydrolysis and condensation of tetraethylorthosilicate(TEOS) leaded) to the formation of organic-inorganic nano-composites. The transmission electron microscope(TEM) showed that the nano-composites with a diameter of about 70 nm were uniform, spherical and mono-dipersed. The nano-composites fluorescence significantly reduced dye leaching and had strong fluorescence when they were by water for several times. Labeled bovine scrum albumin(BSA) with the nano-composites fluorescence showed green fluorescence measured by laser scanning confocal microscope.