CAJ | 학술논문

以1-萘基-3-甲基-5-吡唑啉酮 (NMP)为柱前衍生试剂,探讨了毛细管区带电泳模式下对藏药蕨麻多糖水解液中单糖的分离条件.实验采用58.5 cm×50 μm i.d.毛细管(有效长度50 cm),55 mmol/L硼酸盐缓冲溶液(pH=9.46),柱温20℃,分离电压22 kV,进样10 s.该法不加任何添加剂,9种单糖可高效、快速基线分离,实现了对藏药蕨麻多糖水解液中单糖的分离和定量分析,结果令人满意.
이1-내기-3-갑기-5-필서람동 (NMP)위주전연생시제,탐토료모세관구대전영모식하대장약궐마다당수해액중단당적분리조건.실험채용58.5 cm×50 μm i.d.모세관(유효장도50 cm),55 mmol/L붕산염완충용액(pH=9.46),주온20℃,분리전압22 kV,진양10 s.해법불가임하첨가제,9충단당가고효、쾌속기선분리,실현료대장약궐마다당수해액중단당적분리화정량분석,결과령인만의.
A simple and rapid method was developed for the separation of derivatized carbohydrates of Potentilla anserina L. using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as derivatization reagent by capillary zone electrophoresis. The detection was performed with diode array at 254 nm. A 58.5 cm×50 μm i. d. (50 cm effective length) untreated fused-silica capillary was used. The optimum conditions are as follows:55 mmol/L borate buffer (pH=9.46), voltage 22 kV, column temperature at 20℃ and detection at 254 nm. The samples were atmospherically introduced with an injection time of 10 s. The results indicate that 9 NMP-derivatized carbohydrates achieve baseline resolution within 16 min under the proposed conditions. The Potentilla anserina L. sample was also analyzed under the optimum conditions.