枯草芽孢杆菌SH7抑菌蛋白的分离纯化及对烟草青枯病菌的抑制作用
고초아포간균SH7억균단백적분리순화급대연초청고병균적억제작용
Isolation, Purification and Antagonism of Extracellular Protein from Bacillus subtilis Strain SH7
  • 万方数据
    中国烟草科学 중국연초과학
    CHINESE TOBACCO SCIENCE
    2010年 1期 13-15 ,共3页

    张秀玉%孔凡玉%王静%张成省%李佰乐

    장수옥%공범옥%왕정%장성성%리백악

    枯草芽孢杆菌%抑菌蛋白%纯化%拮抗作用 枯草芽孢桿菌%抑菌蛋白%純化%拮抗作用
    고초아포간균%억균단백%순화%길항작용
    Bacillus subtilis%protein%purification%antagonism activity
    采用硫酸铵分级沉淀、DEAE Sepharose Fast Flow离子交换层析、Sephadex G-75凝胶层析及聚丙烯凝胶电泳,从枯草芽孢杆菌SH7代谢物中分离纯化出一种相对分子质量为33.6 kD的蛋白,该活性物质具有淀粉酶活力,对烟草青枯病菌具有一定的抑制作用.
    채용류산안분급침정、DEAE Sepharose Fast Flow리자교환층석、Sephadex G-75응효층석급취병희응효전영,종고초아포간균SH7대사물중분리순화출일충상대분자질량위33.6 kD적단백,해활성물질구유정분매활력,대연초청고병균구유일정적억제작용.
    Bacillus subtilis strain SH7 showed antagonism against Ralstonia solanacearum.One of the protein was purified by using ammonium sulfate precipitation followed by DEAE Sepharose Fast Flow chromatography and Sephadex G-75.The molecular mass of the protein was 33.6 kD by PAGE analysis.The active protein from Bacillus subtilis strain SH7 have diastatic activity.
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