中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2010年
4期
287-291
,共5页
三七总皂苷%肾间质纤维化%a-平滑肌肌动蛋白%细胞间粘附分子-1
三七總皂苷%腎間質纖維化%a-平滑肌肌動蛋白%細胞間粘附分子-1
삼칠총조감%신간질섬유화%a-평활기기동단백%세포간점부분자-1
Panax notoginseng saponins%renal interstitial fibrosis%asmooth muscle actin:adhesion molecule-1
目的 观察三七总皂苷(抗血栓、抗组织缺血性损伤中药)对肾间质纤维化实验大鼠的肾脏保护作用并探讨其可能的作用机制.方法 用腺嘌呤灌胃法建立大鼠肾间质纤维化模型,将实验动物随机分为2组:实验组(三七总皂苷,35mg·kg~(-1)·d~(-1),灌服4周)和模型组(灌服等量生理盐水),并设立空白对照组.于开始实验第4,7周末,观察大鼠血清细胞间粘附分子-1(ICAM-1)、24 h尿蛋白变化和a-平滑肌肌动蛋白(a-SMA)的表达.结果 实验组、模型组的血清ICAM-1、24 h尿蛋白定量、a-SMA浓度及肾问质纤维化程度等均高于对照组(P＜0.05);但实验组低于模型组(P＜0.05).结论 三七总皂苷可能通过下调模型大鼠a-SMA及ICAM-1的表达,防治肾小管问质纤维化.
目的 觀察三七總皂苷(抗血栓、抗組織缺血性損傷中藥)對腎間質纖維化實驗大鼠的腎髒保護作用併探討其可能的作用機製.方法 用腺嘌呤灌胃法建立大鼠腎間質纖維化模型,將實驗動物隨機分為2組:實驗組(三七總皂苷,35mg·kg~(-1)·d~(-1),灌服4週)和模型組(灌服等量生理鹽水),併設立空白對照組.于開始實驗第4,7週末,觀察大鼠血清細胞間粘附分子-1(ICAM-1)、24 h尿蛋白變化和a-平滑肌肌動蛋白(a-SMA)的錶達.結果 實驗組、模型組的血清ICAM-1、24 h尿蛋白定量、a-SMA濃度及腎問質纖維化程度等均高于對照組(P＜0.05);但實驗組低于模型組(P＜0.05).結論 三七總皂苷可能通過下調模型大鼠a-SMA及ICAM-1的錶達,防治腎小管問質纖維化.
목적 관찰삼칠총조감(항혈전、항조직결혈성손상중약)대신간질섬유화실험대서적신장보호작용병탐토기가능적작용궤제.방법 용선표령관위법건립대서신간질섬유화모형,장실험동물수궤분위2조:실험조(삼칠총조감,35mg·kg~(-1)·d~(-1),관복4주)화모형조(관복등량생리염수),병설립공백대조조.우개시실험제4,7주말,관찰대서혈청세포간점부분자-1(ICAM-1)、24 h뇨단백변화화a-평활기기동단백(a-SMA)적표체.결과 실험조、모형조적혈청ICAM-1、24 h뇨단백정량、a-SMA농도급신문질섬유화정도등균고우대조조(P＜0.05);단실험조저우모형조(P＜0.05).결론 삼칠총조감가능통과하조모형대서a-SMA급ICAM-1적표체,방치신소관문질섬유화.
Objective To observe the protective effects and mechanism of Panax notoginseng saponins(PNS)on the renal interstitial fibrosis rats.Methods Adenine gavage method used rat model of renal fibrosis,the experimental animals were divided into the test group(PNS,dose of 35 mg·kg~(-1)·d~(-1),fed for 4 weeks)and model group,and the establishment of the lank normal group.In the model after 4,7 weeks,the serum cell adhesion molecule-1(ICAM-1),24-hour urine protein auantitative changes and a-smooth muscle actin(a-SMA)were observed.Results In test and model group,the serum ICAM-1,24-hour urine protein,renal a-SMA in the concentration level and renal fibrosis and other indicators higher than the normal group(P＜0.05),but test group lower model group(P＜0.05).Conclusion Panax notoginseng saponins might inhibit tubuloin terstitial fibrosis in adenine nephropathy rat model by suppressing the level of ICAM-1 in serum and the expression of a-SMA in the tubulointerstitial.
