CAJ | 학술논문

肿瘤特异性启动子可驱动目的基因在肿瘤细胞中高效特异性表达,目前常用的有甲胎蛋白启动子、癌胚抗原启动子、前列腺特异性抗原启动子、人端粒酶逆转录酶启动子和多药耐药基因启动子等.利用双启动子、增强子、调控元件或其他物化因素对启动子进行修饰或改造,可增强目的基因在肿瘤细胞中的表达和定位.肿瘤特异性启动子可耦联自杀基因、抑癌基因、反义核酸、细胞凋亡基因和BNA干扰等,在肿瘤基因靶向治疗中发挥重要作用.
종류특이성계동자가구동목적 기인재종류세포중고효특이성표체,목전상용적유갑태단백계동자、암배항원계동자、전렬선특이성항원계동자、인단립매역전록매계동자화다약내약기인계동자등.이용쌍계동자、증강자、조공원건혹기타물화인소대계동자진행수식혹개조,가증강목적 기인재종류세포중적표체화정위.종류특이성계동자가우련자살기인、억암기인、반의핵산、세포조망기인화BNA간우등,재종류기인파향치료중발휘중요작용.
Tumor-specific promoters can induce high-efficiency and specific expression of exogenous genes in tumor cells. At present, commonly used promoters include alpha-fetoprotein promoter, carcinoembryonic antigen promoter,prostate specific antigen promoter,human telomerase reverse transcriptase promoter and multidrug resistance gene promoter and etc. Dual promoters, enhancer, regulatory element and other physical or chem-ical factors could be used to reconstruct or modify promoters to increase the expression and location of exogenous genes in tumor cells. Tumor-specific promoters play an important role in cancer gene targeted therapy by cou-pling with suicide gene, anti- oncogene, antiseuse nucleic acid, apoptosis gene and RNA interference.