CAJ | 학술논문

目的应用实时荧光定量RT-PCR方法检测肺癌外周血中细胞角蛋白19(CK19)mRNA的表达情况,并探讨其表达的临床意义.方法应用实时荧光定量RT-PCR方法检测98例肺癌患者(肺癌组)外周血CK19 mRNA的表达情况,并与28例肺良性疾病患者(肺良性疾病组)和20例健康献血者(对照组)比较.结果肺癌组患者CK19 mRNA阳性率为77.55%(76/98),对照组为0(0/20),肺良性疾病组为3.57%(1/28),三组CK19 mRNA阳性率比较差异有统计学意义(x2=73.680,P＜0.01).肺癌组腺癌患者CK19 mRNA阳性率与鳞癌患者比较差异无统计学意义(x2=0.414,P＞0.05),淋巴结转移阳性患者CK19 mRNA阳性率与阴性患者比较差异有统计学意义(x2=17.523,P＜0.01),Ⅰ期、Ⅱ期和Ⅲ期患者CK19 mRNA阳性率比较差异有统计学意义(x2=13.453,P＜0.01).结论肺癌患者外周血中CK19 mRNA表达较高可能与肺癌转移有关,检测CK19mRNA对于早期判断肺癌转移和复发有一定的临床意义.
목적 응용실시형광정량RT-PCR방법검측폐암외주혈중세포각단백19(CK19)mRNA적표체정황,병탐토기표체적림상의의.방법 응용실시형광정량RT-PCR방법검측98례폐암환자(폐암조)외주혈CK19 mRNA적표체정황,병여28례폐량성질병환자(폐량성질병조)화20례건강헌혈자(대조조)비교.결과 폐암조환자CK19 mRNA양성솔위77.55%(76/98),대조조위0(0/20),폐량성질병조위3.57%(1/28),삼조CK19 mRNA양성솔비교차이유통계학의의(x2=73.680,P＜0.01).폐암조선암환자CK19 mRNA양성솔여린암환자비교차이무통계학의의(x2=0.414,P＞0.05),림파결전이양성환자CK19 mRNA양성솔여음성환자비교차이유통계학의의(x2=17.523,P＜0.01),Ⅰ기、Ⅱ기화Ⅲ기환자CK19 mRNA양성솔비교차이유통계학의의(x2=13.453,P＜0.01).결론 폐암환자외주혈중CK19 mRNA표체교고가능여폐암전이유관,검측CK19mRNA대우조기판단폐암전이화복발유일정적림상의의.
Objective To evaluate the clinical significance of cytokeratin19(CK19) mRNA in the peripheral blood of patients with non-small cell lung cancer (NSCLC). Methods The expression of CK19 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in the peripheral blood from 98 NSCLC patients (NSCLC group), 20 benign pulmonary lesion patients (benign pulmonary lesion group) and 20 healthy adults(control group). Results The positive rate of CK19 mRNA in peripheral blood of NSCLC group, benign pulmonary lesion group and control group was 77.55 % (76/98), 3.57% (1/28),0 (0/20) respectively,and there was significant difference among three groups ( x2 = 73.680,P ＜ 0.01 ). The positive rate of CK19 mRNA in different pathological types of NSCLC had no significant difference (x2 =0.414, P ＞ 0.05 ), but had significant difference in different lymphy node metastasis status( x2 = 17.523, P ＜0.01 ) and different clinical stage ( x2 =13.453,P ＜ 0.01 ). Conclusions The higher expression of CK19mRNA in peripheral blood of NSCLC may relate to cancer metastasis. Detection of CK19 mRNA has significance in early prediction of its prognosis.