中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
2期
223-226
,共4页
邹磊%刘丹彦%雷蕾%吴会生
鄒磊%劉丹彥%雷蕾%吳會生
추뢰%류단언%뢰뢰%오회생
高压氧%缺血预处理%再灌注损伤%脑%老年人%Nogo%Nogo-A%NgR
高壓氧%缺血預處理%再灌註損傷%腦%老年人%Nogo%Nogo-A%NgR
고압양%결혈예처리%재관주손상%뇌%노년인%Nogo%Nogo-A%NgR
Hyperbaric oxygenation%Ischemic preconditioning%Reperfusion injury%Brain%Aged%Nogo%Nogo-A%NgR
目的 研究高压氧预处理对老龄大鼠全脑缺血再灌注损伤时Nogo mRNA、Nogo-A及NgR蛋白表达的影响,探讨其改善认知功能的可能机制.方法 雄性SD大鼠42只,月龄14月,随机分为4组:对照组(C组,n=6)、高压氧组(H组,n=12)、脑缺血再灌注损伤组(I/R组,n=12)和高压氧预处理组(HOP组,n=12).H组和HOP组每天置于高压氧舱内1h,氧压为0.2 MPa,连续5 d,最后1次高压氧处理后24 h时I/R组和HOP组采用改良Pulsinelli四血管闭塞法制备大鼠全脑缺血再灌注损伤模型,全脑缺血10 min.再灌注24 h时H组、I/R组和HOP组随机取6只大鼠断头取脑,分离大脑皮质,采用实时荧光定量PCR法检测Nogo mRNA的表达水平,Western blot法检测Nogo-A及NgR蛋白的表达水平.余大鼠自由喂养5 d后采用Morris水迷宫实验测定认知功能.结果 与C组比较,I/R组和HOP组认知功能降低,Nogo mRNA及Nogo-A蛋白表达上调(P＜0.05);与I/R组比较,H组和HOP组认知功能提高,Nogo mRNA及Nogo-A蛋白表达下调(P＜0.05).各组NgR蛋白表达比较差异无统计学意义(P＞0.05).结论 高压氧预处理通过抑制脑皮质Nogo mRNA及Nogo-A蛋白表达上调,改善老龄大鼠全脑缺血再灌注损伤时的认知功能.
目的 研究高壓氧預處理對老齡大鼠全腦缺血再灌註損傷時Nogo mRNA、Nogo-A及NgR蛋白錶達的影響,探討其改善認知功能的可能機製.方法 雄性SD大鼠42隻,月齡14月,隨機分為4組:對照組(C組,n=6)、高壓氧組(H組,n=12)、腦缺血再灌註損傷組(I/R組,n=12)和高壓氧預處理組(HOP組,n=12).H組和HOP組每天置于高壓氧艙內1h,氧壓為0.2 MPa,連續5 d,最後1次高壓氧處理後24 h時I/R組和HOP組採用改良Pulsinelli四血管閉塞法製備大鼠全腦缺血再灌註損傷模型,全腦缺血10 min.再灌註24 h時H組、I/R組和HOP組隨機取6隻大鼠斷頭取腦,分離大腦皮質,採用實時熒光定量PCR法檢測Nogo mRNA的錶達水平,Western blot法檢測Nogo-A及NgR蛋白的錶達水平.餘大鼠自由餵養5 d後採用Morris水迷宮實驗測定認知功能.結果 與C組比較,I/R組和HOP組認知功能降低,Nogo mRNA及Nogo-A蛋白錶達上調(P＜0.05);與I/R組比較,H組和HOP組認知功能提高,Nogo mRNA及Nogo-A蛋白錶達下調(P＜0.05).各組NgR蛋白錶達比較差異無統計學意義(P＞0.05).結論 高壓氧預處理通過抑製腦皮質Nogo mRNA及Nogo-A蛋白錶達上調,改善老齡大鼠全腦缺血再灌註損傷時的認知功能.
목적 연구고압양예처리대노령대서전뇌결혈재관주손상시Nogo mRNA、Nogo-A급NgR단백표체적영향,탐토기개선인지공능적가능궤제.방법 웅성SD대서42지,월령14월,수궤분위4조:대조조(C조,n=6)、고압양조(H조,n=12)、뇌결혈재관주손상조(I/R조,n=12)화고압양예처리조(HOP조,n=12).H조화HOP조매천치우고압양창내1h,양압위0.2 MPa,련속5 d,최후1차고압양처리후24 h시I/R조화HOP조채용개량Pulsinelli사혈관폐새법제비대서전뇌결혈재관주손상모형,전뇌결혈10 min.재관주24 h시H조、I/R조화HOP조수궤취6지대서단두취뇌,분리대뇌피질,채용실시형광정량PCR법검측Nogo mRNA적표체수평,Western blot법검측Nogo-A급NgR단백적표체수평.여대서자유위양5 d후채용Morris수미궁실험측정인지공능.결과 여C조비교,I/R조화HOP조인지공능강저,Nogo mRNA급Nogo-A단백표체상조(P＜0.05);여I/R조비교,H조화HOP조인지공능제고,Nogo mRNA급Nogo-A단백표체하조(P＜0.05).각조NgR단백표체비교차이무통계학의의(P＞0.05).결론 고압양예처리통과억제뇌피질Nogo mRNA급Nogo-A단백표체상조,개선노령대서전뇌결혈재관주손상시적인지공능.
Objective To investigate the effect of hyperbaric oxygen preconditioning(HOP)on expression of Nogo mRNA,Nogo-A and NgR protein in the cerebral cortex after acute global cerebral ischemia-reperfusion(I/R)injury in aged rats.Methods Forty-two aged male SD rats aged 14 months weighing 480-530 g were randomly divided into 4 groups: group Ⅰ control(group C,n = 6);group Ⅱ HOP(n = 12);group Ⅲ cerebral I/R(n = 12)and group Ⅳ HOP+I/R(n = 12).Global cerebral I/R was induced by 4-vesasl occlusion method described by Pulsinelli.Bilateral vertebral arteries were permanently occluded by cauterization,aad bilateral carotid arteries were occluded for 10 min.Successful induction of cerebral ischemia was confirmed by bilateral dilated pupils unresponsive to light and EEG changes.In group Ⅱ and Ⅳ the animals received 1 h hyperbaric oxyyen at 2 absolute atmospheric pressure per day for 5 consecutive days.In group Ⅳ(HOP+I/R)global cerebral I/R was induced at 24 h after last hyperbaric oxyyen treatment.Six animals in each group were killed at24 h after I/R in each group and their brains were removed for detection of Nogo mRNA expression(by Q-PCR)and expression of Nago-A and NgR protein(by Western blot)in cerebral cortex.Cognitive function was determined at 5 days after cerebral ischemia using Morris Water Maze in the other animals.Results The learning ability and memory were significantly decreased while the expression of Nogo mRNA and Nogo-A protein was significantly increased in group Ⅲ(I/R)and Ⅳ(HOP+I/R)as compared with control group.HOP significantly ameliorated the cerebral I/R-induced decrease in learning ability and memory and increase in Nogo mRNA and Nogo-A protein expression.Conclusion Hyperbaric oxyyen preconditioning can ameliorate acute global cerebral I/R injury-induced cognitive impairment by decreasing Nogo mRNA and Nogo-A protein expression in the brain.
