中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
5期
618-621
,共4页
石旺%胡成平%田科%肖志科
石旺%鬍成平%田科%肖誌科
석왕%호성평%전과%초지과
异硫氰酸盐类/药理学%小细胞肺癌/代谢/病理学%细胞系,肿瘤%细胞增殖%肿瘤侵润%基质金属蛋白酶9/代谢
異硫氰痠鹽類/藥理學%小細胞肺癌/代謝/病理學%細胞繫,腫瘤%細胞增殖%腫瘤侵潤%基質金屬蛋白酶9/代謝
이류청산염류/약이학%소세포폐암/대사/병이학%세포계,종류%세포증식%종류침윤%기질금속단백매9/대사
Isothiocyanates/pharmacology%Small cell lung carcinoma/metabolism/pathology%Cell line,tumor%Cell proliferation%Neoplasm invasiveness%Matrix metalloproteinase 9/metabolism
目的 探讨莱菔硫烷(sulforaphane,SFN)对人小细胞肺癌NCI-H446细胞增殖、侵袭以及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)活性的影响.方法 体外培养NCI-H446细胞,用0、25、50、100 μmol/L SFN处理24 ～72 h后,MTT法检测细胞的增殖情况；小室侵袭实验分析细胞的侵袭力改变,明胶酶谱实验检测MMP-9的酶活性变化.结果 经25、50、100 μmol/L SFN处理24～72 h后,NCI-H446生长均能受到不同程度抑制.其中72 h后,25、50、100 μmol/L SFN组NCI-H446细胞的抑制率分别为(11.1±2.26)％,(25.2±3.24)％和(44.6±4.2)％,与溶剂对照组相比差异具有统计学意义(t =10.685,8.417,5.264,P＜0.05).25、50、100 μmol/L SFN作用NCIH446细胞24h后,NCI-H446细胞穿膜数分别为(48.6±1.84)％,(35.4±2.22)％和(27.8±1.36)％,与溶剂对照组[(68.4±2.21)％]相比差异具有统计学意义(t =6.341,5.562,4.925,P＜0.05).明胶酶谱实验显示,25、50、100 μmol/L SFN处理后,MMP-9活性的灰度值分别为764±18.4,685±14.74和638±21.54,与对照组相比(822±12.53),差异有统计学意义(t=4.971,7.582,11.235,P＜0.05).结论 SFN对肺癌NCI-H446细胞生长、侵袭力及MMP-9的活性具有抑制作用.
目的 探討萊菔硫烷(sulforaphane,SFN)對人小細胞肺癌NCI-H446細胞增殖、侵襲以及基質金屬蛋白酶-9(matrix metalloproteinase-9,MMP-9)活性的影響.方法 體外培養NCI-H446細胞,用0、25、50、100 μmol/L SFN處理24 ～72 h後,MTT法檢測細胞的增殖情況；小室侵襲實驗分析細胞的侵襲力改變,明膠酶譜實驗檢測MMP-9的酶活性變化.結果 經25、50、100 μmol/L SFN處理24～72 h後,NCI-H446生長均能受到不同程度抑製.其中72 h後,25、50、100 μmol/L SFN組NCI-H446細胞的抑製率分彆為(11.1±2.26)％,(25.2±3.24)％和(44.6±4.2)％,與溶劑對照組相比差異具有統計學意義(t =10.685,8.417,5.264,P＜0.05).25、50、100 μmol/L SFN作用NCIH446細胞24h後,NCI-H446細胞穿膜數分彆為(48.6±1.84)％,(35.4±2.22)％和(27.8±1.36)％,與溶劑對照組[(68.4±2.21)％]相比差異具有統計學意義(t =6.341,5.562,4.925,P＜0.05).明膠酶譜實驗顯示,25、50、100 μmol/L SFN處理後,MMP-9活性的灰度值分彆為764±18.4,685±14.74和638±21.54,與對照組相比(822±12.53),差異有統計學意義(t=4.971,7.582,11.235,P＜0.05).結論 SFN對肺癌NCI-H446細胞生長、侵襲力及MMP-9的活性具有抑製作用.
목적 탐토래복류완(sulforaphane,SFN)대인소세포폐암NCI-H446세포증식、침습이급기질금속단백매-9(matrix metalloproteinase-9,MMP-9)활성적영향.방법 체외배양NCI-H446세포,용0、25、50、100 μmol/L SFN처리24 ～72 h후,MTT법검측세포적증식정황；소실침습실험분석세포적침습력개변,명효매보실험검측MMP-9적매활성변화.결과 경25、50、100 μmol/L SFN처리24～72 h후,NCI-H446생장균능수도불동정도억제.기중72 h후,25、50、100 μmol/L SFN조NCI-H446세포적억제솔분별위(11.1±2.26)％,(25.2±3.24)％화(44.6±4.2)％,여용제대조조상비차이구유통계학의의(t =10.685,8.417,5.264,P＜0.05).25、50、100 μmol/L SFN작용NCIH446세포24h후,NCI-H446세포천막수분별위(48.6±1.84)％,(35.4±2.22)％화(27.8±1.36)％,여용제대조조[(68.4±2.21)％]상비차이구유통계학의의(t =6.341,5.562,4.925,P＜0.05).명효매보실험현시,25、50、100 μmol/L SFN처리후,MMP-9활성적회도치분별위764±18.4,685±14.74화638±21.54,여대조조상비(822±12.53),차이유통계학의의(t=4.971,7.582,11.235,P＜0.05).결론 SFN대폐암NCI-H446세포생장、침습력급MMP-9적활성구유억제작용.
Objective To investigate the effects of sulforaphane (SFN) on proliferation and invasion of human small cell lung cancer NCI-H446 and the activity of matrix metalloproteinase (MMP) -9.Methods NCI-H446 cells were cultured with 0,25,50,100 μmol/L SFN for 24 ～ 72 h,then MTT assay was employed to detect cell proliferation.Chamber invasion assay was used to study the cell invasion,and gelatin zymography assay was implied in MMP-9 enzyme activity.Results After treatment of 25,50,100μmol/L SFN,the growth of NCI-H446 cells were inhibited.When cells were incubated with 25,50,100μmol/L of SFN for 72h,the inhibition ratio was ( 11.1 ± 2.26 ) ％,( 25.2 ± 3.24 ) ％ and ( 44.6 ±4.2) ％,respectively,the difference was statistically significant compared with the solvent control group ( t =10.685,8.417,5.264,P ＜0.05 ).Chamber invasion assay showed that NCI-H446 cell invasion could be reduced.25,50,100 μmol/L of SFN could decrease the trans-membrane cells to (48.6 ± 1.84)％,(35.4 ± 2.22) ％ and (27.8 ± 1.36) ％,and it was statistically significant compared with the solvent control group ( t =6.341,5.562,4.925,P ＜0.05 ),respectively.In addition,MMP-9 activity was significantly inhibited by SFN.25,50,100 μmol/L of SFN could decrease the gray value of MMP-9 to 764 ±18.4,685 ± 14.74 and 638 ± 21.54 ( control group 822 ± 12.53,t =4.971,7.582,11.235,respectively,P ＜0.05).Conclusions SFN can inhibit NCI-H446 cells growth,invasion and the activity ofMMP-9.
