CAJ | 학술논문

目的探讨缺氧诱导因子-1α(HIF-1α)及转录异常对肝癌的临床价值.方法以肝癌模型观察肝病理学、HIF-1α转录及表达水平的动态变化;以自身配对法收集手术切除的肝癌和癌周组织,抽提总RNA并扩增分析HIF-1α mRNA表达;以免疫组织化学和Western blot分析HIF-1α表达、胞内分布及临床病理学特征.根据不同资料采用方差分析、确切概率法、Pearson或Spearman等级相关分析进行统计学分析. 结果癌变过程中肝HIF-1α仅和HIF-1αmRNA均呈梯度表达.在对照组、变性组、癌前组和癌变组中,肝HIF-1α阳性率为0、77.8%、88.90/o和100%;HIF-1α/β-肌动蛋白比值为0.16±0.02、0.29±0.04、0.52±0.03和0.84±0.02,均显著高于对照组(P=0.000).人肝癌组织HIF-1α阳性率为80.0%(28/35),癌旁为100%(35/35),且与瘤体大小呈正相关,与分化程度呈负相关,与肿瘤数目及HBsAg阳性无关.结论 HIF-1表达与肝癌发生、发展相关,是肝癌治疗的分子靶目标.
목적 탐토결양유도인자-1α(HIF-1α)급전록이상대간암적림상개치.방법 이간암모형관찰간병이학、HIF-1α전록급표체수평적동태변화;이자신배대법수집수술절제적간암화암주조직,추제총RNA병확증분석HIF-1α mRNA표체;이면역조직화학화Western blot분석HIF-1α표체、포내분포급림상병이학특정.근거불동자료채용방차분석、학절개솔법、Pearson혹Spearman등급상관분석진행통계학분석. 결과암변과정중간HIF-1α부화HIF-1αmRNA균정제도표체.재대조조、변성조、암전조화암변조중,간HIF-1α양성솔위0、77.8%、88.90/o화100%;HIF-1α/β-기동단백비치위0.16±0.02、0.29±0.04、0.52±0.03화0.84±0.02,균현저고우대조조(P=0.000).인간암조직HIF-1α양성솔위80.0%(28/35),암방위100%(35/35),차여류체대소정정상관,여분화정도정부상관,여종류수목급HBsAg양성무관.결론 HIF-1표체여간암발생、발전상관,시간암치료적분자파목표.
Objective To investigate the dynamic expression of hypoxia inducible factor-1α(HIF1α) and its clinical values in hepotacellular carcinoma(HCC).Methods The dynamic changes of liver pathology,HIF-1α transcription and expression were observed through the hepatoma model.The self-control specimens from 35 human HCC patients were collected and the expression,cellular distribution,and clinopathological features of HIF-1α and its gene was analyzed by immohistochemistry,western blotting and nested-PCR,respectively.Results Both levels of hepatic HIF-1α and HIF-1α mRNA expression increased during the HCC development course.The incidence of HIF-1α and the ratio of HIF-1α to β-actin was 0% and 0.16±0.02 in the control rats,77.8% and 0.29±0.04 in the denatured rats,88.9% and 0.52±0.03 in the precancerous rats,and 100% and 0.84±0.02 in the cancerous rats respectively,with significant difference between the control group and any of the experimental groups(P=0.000).The positive HIF-1α was brown and granule-like and mainly presented in cytoplasm and few in nucleus.The incidence of HIF-1α was 80%(28/35)in HCC and 100%(35/35)in its surrounding tissues.The clinical pathological features indicated HIF-1α expression associated with tumor size and differentiation degree the of HCC.No correlation was found between HIF-1 α and tumor numbers or positive-Hbsag.Conclusions HIF-1α expression is associated with occurrence and development of HCC,and is perpahps a target molecule for HCC therapy.