CAJ | 학술논문

目的探讨维生素D受体(VDR)基因Fok Ⅰ位点F/f单核苷酸多态性(SNP)与Ⅲ型前列腺炎的关系.方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对103例Ⅲ型前列腺炎患者(A组)及100例非前列腺疾病患者(B组)进行Fok Ⅰ位点SNP检测,分析两组间基因型和等位基因的频率分布.采用Logistic多元回归法分析Fok Ⅰ位点SNP与Ⅲ型前列腺炎的相关性.结果 VDR基因Fok Ⅰ位点基因型分布在A组及B组中均符合Hardy-Weinberg平衡.A组基因型分布FF:28％ (29/103),Ff:31％ (32/103),ff:41％ (42/103)；B组FF:31％ (31/100),Ff:35％(35/100),ff:34％ (34/100),其中ff基因型分布在两组中的差异有统计学意义(P＜0.05).A组等位基因频率分布F:44％( 90/206),f:56％ (116/206)；B组为F:49％ (97/200),f:51％ (103/200),分布频率差异有统计学意义(P＜0.05).Logistic回归分析结果显示,Fok Ⅰ多态性是Ⅲ型前列腺炎发生的独立风险因素(P＜0.05).结论 VDR基因Fok Ⅰ位点SNP与Ⅲ型前列腺炎的发生有一定关系.
목적 탐토유생소D수체(VDR)기인Fok Ⅰ위점F/f단핵감산다태성(SNP)여Ⅲ형전렬선염적관계.방법 응용취합매련반응-한제성편단장도다태성(PCR-RFLP)대103례Ⅲ형전렬선염환자(A조)급100례비전렬선질병환자(B조)진행Fok Ⅰ위점SNP검측,분석량조간기인형화등위기인적빈솔분포.채용Logistic다원회귀법분석Fok Ⅰ위점SNP여Ⅲ형전렬선염적상관성.결과 VDR기인Fok Ⅰ위점기인형분포재A조급B조중균부합Hardy-Weinberg평형.A조기인형분포FF:28％ (29/103),Ff:31％ (32/103),ff:41％ (42/103)；B조FF:31％ (31/100),Ff:35％(35/100),ff:34％ (34/100),기중ff기인형분포재량조중적차이유통계학의의(P＜0.05).A조등위기인빈솔분포F:44％( 90/206),f:56％ (116/206)；B조위F:49％ (97/200),f:51％ (103/200),분포빈솔차이유통계학의의(P＜0.05).Logistic회귀분석결과현시,Fok Ⅰ다태성시Ⅲ형전렬선염발생적독립풍험인소(P＜0.05).결론 VDR기인Fok Ⅰ위점SNP여Ⅲ형전렬선염적발생유일정관계.
Objective To investigate the association between F/f single nucleotide polymorphism (SNP) of Fok Ⅰ in vitamin D receptor (VDR) gene and type Ⅲ prostatitis.Methods In 103 patients with type Ⅲ prostatitis (group A) and 100 control patients without prostatitis (group B),VDR gene Fok ⅠSNP was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The distribution of genotype and allelic frequencies were analyzed.Multiple Logistic analysis was used to examine the association between SNP of Fok Ⅰ and type Ⅲ prostatitis.Results The genotype distribution of VDR gene FokⅠ was in Hardy-Weinberg equilibrium in both two groups.Genotype distribution of FF,Ff and ff in group A was 28％ (29/103),31％ (32/103) and 41％ (42/103) respectively,and 31％ (31/100),35％ (35/100),34％ (34/100) respectively in group B.There was significant difference between two groups in frequency distribution of ff genotype (P ＜ 0.05).The distribution of allelic frequencies of F and f was 44％ (90/206) and 56％ (116/206) in group A,and 49％ (97/200),51％ (103/200) in group B,respectively.There was significant difference between two groups in frequency distribution of allele (P ＜0.05).The FokⅠ polymorphism was an independent risk factor with type Ⅲ prostatitis by multiple Logistic analysis (P ＜ 0.05).Conclusion VDR FokⅠpolymorphisms are correlated with type Ⅲ prostatitis.