中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
42期
2980-2983
,共4页
郭正辉%黄海%杜涛%许可慰%曹亿%陈杰青%董文%姚友生%林天歆%谢文练%江春%韩金利%黄健
郭正輝%黃海%杜濤%許可慰%曹億%陳傑青%董文%姚友生%林天歆%謝文練%江春%韓金利%黃健
곽정휘%황해%두도%허가위%조억%진걸청%동문%요우생%림천흠%사문련%강춘%한금리%황건
前列腺肿瘤%色素上皮衍生因子%转移%免疫组化
前列腺腫瘤%色素上皮衍生因子%轉移%免疫組化
전렬선종류%색소상피연생인자%전이%면역조화
Prostate neoplasms%Pigment epithelium-derived factor%Metastasis%Immunohistochemistry
目的 观察色素上皮衍生因子(PEDF)在前列腺癌发生发展中对前列腺癌抑制的作用.方法 通过免疫组化S-P法及Western印迹检测正常前列腺组织、良性前列腺增生组织、前列腺癌组织以及前列腺癌细胞系PC-3、Lncap中PEDF的表达情况,结合临床资料进行统计分析,评价PEDF表达情况与前列腺组织癌变之间的关系;同时联系前列腺癌不同病理分级,并研究其与PEDF表达之间的关系.结果 在正常前列腺组织及良性前列腺组织中PEDF均处于高表达状态,远远高于前列腺癌组织及前列腺癌细胞系中PEDF的表达[7例正常前列腺组织中7例PEDF阳性表达;20例良性前列腺增生组织19例PEDF阳性表达;83例前列腺癌组织中24例(28.9%)PEDF阳性表达].PEDF的表达情况与前列腺癌病理分级相关,低分化的前列腺癌组织中PEDF的表达量低于高分化的前列腺癌组织.在转移的前列腺癌组织中PEDF的表达率为12%,低于未转移的前列腺癌组织中的43.1%.结论 PEDF与前列腺癌的发生呈负相关,PEDF表达量越低前列腺癌细胞的恶性度越高,越具有转移侵袭的能力.
目的 觀察色素上皮衍生因子(PEDF)在前列腺癌髮生髮展中對前列腺癌抑製的作用.方法 通過免疫組化S-P法及Western印跡檢測正常前列腺組織、良性前列腺增生組織、前列腺癌組織以及前列腺癌細胞繫PC-3、Lncap中PEDF的錶達情況,結閤臨床資料進行統計分析,評價PEDF錶達情況與前列腺組織癌變之間的關繫;同時聯繫前列腺癌不同病理分級,併研究其與PEDF錶達之間的關繫.結果 在正常前列腺組織及良性前列腺組織中PEDF均處于高錶達狀態,遠遠高于前列腺癌組織及前列腺癌細胞繫中PEDF的錶達[7例正常前列腺組織中7例PEDF暘性錶達;20例良性前列腺增生組織19例PEDF暘性錶達;83例前列腺癌組織中24例(28.9%)PEDF暘性錶達].PEDF的錶達情況與前列腺癌病理分級相關,低分化的前列腺癌組織中PEDF的錶達量低于高分化的前列腺癌組織.在轉移的前列腺癌組織中PEDF的錶達率為12%,低于未轉移的前列腺癌組織中的43.1%.結論 PEDF與前列腺癌的髮生呈負相關,PEDF錶達量越低前列腺癌細胞的噁性度越高,越具有轉移侵襲的能力.
목적 관찰색소상피연생인자(PEDF)재전렬선암발생발전중대전렬선암억제적작용.방법 통과면역조화S-P법급Western인적검측정상전렬선조직、량성전렬선증생조직、전렬선암조직이급전렬선암세포계PC-3、Lncap중PEDF적표체정황,결합림상자료진행통계분석,평개PEDF표체정황여전렬선조직암변지간적관계;동시련계전렬선암불동병리분급,병연구기여PEDF표체지간적관계.결과 재정상전렬선조직급량성전렬선조직중PEDF균처우고표체상태,원원고우전렬선암조직급전렬선암세포계중PEDF적표체[7례정상전렬선조직중7례PEDF양성표체;20례량성전렬선증생조직19례PEDF양성표체;83례전렬선암조직중24례(28.9%)PEDF양성표체].PEDF적표체정황여전렬선암병리분급상관,저분화적전렬선암조직중PEDF적표체량저우고분화적전렬선암조직.재전이적전렬선암조직중PEDF적표체솔위12%,저우미전이적전렬선암조직중적43.1%.결론 PEDF여전렬선암적발생정부상관,PEDF표체량월저전렬선암세포적악성도월고,월구유전이침습적능력.
Objective To study the inhibition and significance of pigment epithelium-derived factor (PEDF) in the development and metastasis of prostate cancer. Methods The expression of PEDF was examined in the normal prostate tissue, benign prostatic hyperplasia, prostate cancer tissue and prostate cancer cell lines, PC-3 and Lncap by immunohistochemical SP method and Western blot In combination with clinical data, statistical analysis was performed to evaluate the relation of the expression level of PEDF in prostate cancer and the relationship between different histological grades of prostate cancer. Results In normal prostate tissue and benign prostate tissue, the expression of PEDF were elevated and it was far higher than the prostate cancer and prostate cancer cell line. The expression of PEDF and the pathological grade of prostate cancer were related to the differentiated carcinoma of prostate tissue, and the expression level of PEDF in poorly differentiated carcinoma below the highly differentiated carcinoma of prostate cancer. In metastatic prostate cancer, the expression of PEDF was lower than that of prostate cancer without metastasis (12% vs 43. 1%). Conclusion The expression of PEDF and the incidence of prostate cancer have a negative correlation. The lower grade of prostate cancer cells, the less the tissue expression of PEDF and the higher tendency of invasion and metastasis.
