CAJ | 학술논문

目的探讨高压氧(HBO)治疗缺氧缺血性脑损伤新生大鼠后,内源性神经干细胞增殖的动态变化.方法将7日龄Sprague-Dawley新生大鼠随机分为正常对照组(对照组)、缺氧缺血脑损伤组(模型组)与HBO治疗组(HBO组).采用经典的Rice-Vannucci法制作缺氧缺血性脑损伤模型,HBO组在脑损伤后3 h内进行HBO治疗,治疗压力为2个绝对大气压,稳压60 min,每日1次,连续7 d.分别于HBO治疗后第3小时、第21小时、第3天、第7天和第14天,采用5-溴-2-脱氧脲苷(BrdU)/巢蛋白免疫荧光双重标记法动态检测缺血侧侧脑室室管膜下区与海马齿状回内源性神经干细胞增殖的动态变化;采用Western blot法检测缺血侧大脑半球巢蛋白的动态变化.结果 HBO组室管膜下区与海马齿状回BrdU +/巢蛋白标记的神经干阳性细胞数分别于HBO治疗后第3小时及第21小时显著增加,第7天达最高水平,并显著高于模型组和对照组(P<0.01),第14天BrdU +/巢蛋白阳性细胞数开始下降,仍高于模型组和对照组(P<0.01);巢蛋白的Western blot分析发现,缺血侧大脑半球巢蛋白于HBO治疗后第21小时开始增加,第7天达峰值后下降.结论缺氧缺血性脑损伤后3 h内给予HBO治疗,可以促进缺氧缺血性脑损伤新生大鼠内源性神经干细胞的增殖.
목적 탐토고압양(HBO)치료결양결혈성뇌손상신생대서후,내원성신경간세포증식적동태변화.방법 장7일령Sprague-Dawley신생대서수궤분위정상대조조(대조조)、결양결혈뇌손상조(모형조)여HBO치료조(HBO조).채용경전적Rice-Vannucci법제작결양결혈성뇌손상모형,HBO조재뇌손상후3 h내진행HBO치료,치료압력위2개절대대기압,은압60 min,매일1차,련속7 d.분별우HBO치료후제3소시、제21소시、제3천、제7천화제14천,채용5-추-2-탈양뇨감(BrdU)/소단백면역형광쌍중표기법동태검측결혈측측뇌실실관막하구여해마치상회내원성신경간세포증식적동태변화;채용Western blot법검측결혈측대뇌반구소단백적동태변화.결과 HBO조실관막하구여해마치상회BrdU +/소단백표기적신경간양성세포수분별우HBO치료후제3소시급제21소시현저증가,제7천체최고수평,병현저고우모형조화대조조(P<0.01),제14천BrdU +/소단백양성세포수개시하강,잉고우모형조화대조조(P<0.01);소단백적Western blot분석발현,결혈측대뇌반구소단백우HBO치료후제21소시개시증가,제7천체봉치후하강.결론 결양결혈성뇌손상후3 h내급여HBO치료,가이촉진결양결혈성뇌손상신생대서내원성신경간세포적증식.
Objective To study the effect of hyperbaric oxygen (HBO) therapy on changes in the prolifera-tion of endogenous neural stem ceils (NSCs) in neonatal rats after hypoxie ischemic brain damage (HIBD). Meth-ods A total of 150 Sprague-Dawley rats aged 7 d were randomly divided into a normal eontrol group ( CON ), a HIBD group and a HBO treatment group. HBO was administered to the HBO treatment group within 3 h after HIBD at 2 atmospheres, once daily for 7 d. The HIBD model rats were subjected to unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2> ). BrdU/nestin immunofluorescence was used to examine the proliferation of NSCs dynami-cally in the subventricular zone (SVZ) and the dentate gyms (DG) at the 3rd h, 21st h, 3rd d, 7th d and 14th d af-ter HBO therapy. Nestin protein was detected by Western blot analysis at various time points after HIBD. Results In the HIBD rats treated with HBO, proliferation of endogenous NSCs was observed in the SVZ and DG. The prolifer-ating NSCs increased at the 3rd h and 21st h after HBO therapy in the SVZ and DG respectively, peaked at the 7th d after HBO therapy, and decreased by the 14th d after HBO therapy, though their level was still higher than that in the controls. The Western blot analysis showed that nestin protein began to increase at the 21st h after HBO therapy, peaked at the 7th d after HBO therapy, then decreased. Conclusion HBO administered within 3 h after HIBD can promote proliferation of endogenous NSCs in neonatal rats after HIBD.