中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2009年
5期
289-291
,共3页
烧伤%瘢痕%模型%动物
燒傷%瘢痕%模型%動物
소상%반흔%모형%동물
Burns%Scarring%Animal models
目的 对传统的增生性瘢痕动物模型进行改进,为增生性瘢痕发病机制的研究建立更实用的动物模型.方法 将20只裸鼠随机分成观察组和对照组,每组10只.观察组裸鼠背部皮下移植人全厚皮肤,皮片存活后用加热的铜柱造成深Ⅱ度烧伤;对照组裸鼠如同观察组移植人全厚皮片,但未予热烧伤,观察皮片存活、创面愈合与瘢痕增生情况.结果 观察组裸鼠存活9只,移植存活皮片与正常成人皮肤相比,无明显差异;烧伤后有8只可见明显、持续的瘢痕增生,其外观和组织学特点与人体增生性瘢痕相似,组织学观察可见丰富胶原纤维和炎症浸润.对照组存活8只,皮片干痂脱落后,有6只出现类似瘢痕样增生.结论 与以往模型相比,改进后模型的组织来源更可靠,皮片存活率及瘢痕复制率更高,建立周期更短,且瘢痕增生明显,可用于观察创面愈合至瘢痕形成的全过程,因此是一种较理想的研究增生性瘢痕的动物模型.
目的 對傳統的增生性瘢痕動物模型進行改進,為增生性瘢痕髮病機製的研究建立更實用的動物模型.方法 將20隻裸鼠隨機分成觀察組和對照組,每組10隻.觀察組裸鼠揹部皮下移植人全厚皮膚,皮片存活後用加熱的銅柱造成深Ⅱ度燒傷;對照組裸鼠如同觀察組移植人全厚皮片,但未予熱燒傷,觀察皮片存活、創麵愈閤與瘢痕增生情況.結果 觀察組裸鼠存活9隻,移植存活皮片與正常成人皮膚相比,無明顯差異;燒傷後有8隻可見明顯、持續的瘢痕增生,其外觀和組織學特點與人體增生性瘢痕相似,組織學觀察可見豐富膠原纖維和炎癥浸潤.對照組存活8隻,皮片榦痂脫落後,有6隻齣現類似瘢痕樣增生.結論 與以往模型相比,改進後模型的組織來源更可靠,皮片存活率及瘢痕複製率更高,建立週期更短,且瘢痕增生明顯,可用于觀察創麵愈閤至瘢痕形成的全過程,因此是一種較理想的研究增生性瘢痕的動物模型.
목적 대전통적증생성반흔동물모형진행개진,위증생성반흔발병궤제적연구건립경실용적동물모형.방법 장20지라서수궤분성관찰조화대조조,매조10지.관찰조라서배부피하이식인전후피부,피편존활후용가열적동주조성심Ⅱ도소상;대조조라서여동관찰조이식인전후피편,단미여열소상,관찰피편존활、창면유합여반흔증생정황.결과 관찰조라서존활9지,이식존활피편여정상성인피부상비,무명현차이;소상후유8지가견명현、지속적반흔증생,기외관화조직학특점여인체증생성반흔상사,조직학관찰가견봉부효원섬유화염증침윤.대조조존활8지,피편간가탈락후,유6지출현유사반흔양증생.결론 여이왕모형상비,개진후모형적조직래원경가고,피편존활솔급반흔복제솔경고,건립주기경단,차반흔증생명현,가용우관찰창면유합지반흔형성적전과정,인차시일충교이상적연구증생성반흔적동물모형.
Objective To improve the animal model of hypertrophic scars for studying their pathology. Methods Twenty nude mice were divided randomly into two groups of 10. In group one every mouse received a full thickness piece of human skin grafted into the subcutaneous tissue of their hack. After the graft had survived 14d after transplantation, a burn was caused on the graft with a heated copper cylinder. The mice in the second group received the transplant but no burn. The status of the grafts and scars was observed, and histological examinations of the grafts were performed. Results Nine mice in group one survived, of which eight developed obvious and persistent hyper-trophic scars after the burns. These were hard and rose above the surrounding skin. Eight nude mice survived in group two, of which only six developed hypertrophic sear analogues after transplantation. Histological examinations showed abundant collagen deposition and inflammatory infiltration in all sears. Conclusion The similarity, repeat-ability, certainty, and survival rate of this improved hypertrophic scar model are superior to those reported previously. This improved animal model can induce obvious and persistent scarring that can be used to observe the whole proce-dure of hypertrophic scarring, so it is a useful tool for studying hypertrophic scars.
