中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
4期
335-339
,共5页
黄敏婷%陆春%朱国兴%冯佩英%赖维%叶晓敏%林飞燕%郑锦芬%马寒%李美荣
黃敏婷%陸春%硃國興%馮珮英%賴維%葉曉敏%林飛燕%鄭錦芬%馬寒%李美榮
황민정%륙춘%주국흥%풍패영%뢰유%협효민%림비연%정금분%마한%리미영
解脲脲原体%生物膜%胞外多糖
解脲脲原體%生物膜%胞外多糖
해뇨뇨원체%생물막%포외다당
Ureaplasma urealyticum%Biofilm%Exopolysaccharide
目的 研究解脲脲原体(Ureaplasma urealyticum,Uu)4个型别标准株在体外形成的生物膜之胞外多糖的分布及结构成分.方法 将Uu标准株Parvo群中4、8血清型和T960群中3、14血清型进行体外生物膜培养后,扫描电镜下观察生物膜组成及结构,并在FITC-ConA/PI及ECA/PI双荧光染色后进行激光共聚焦显微镜观察及测定平均荧光强度.秩和检验及t检验分别比较两种荧光标记物、两生物群的总体平均荧光强度的差异.结果 4个型别Uu标准株均可在体外形成生物膜,生物膜结构主要呈网格状,胞外物质占大部分比例.在激光共聚焦显微镜下,Uu生物膜胞外多糖均可被FITC-ConA和ECA染色,FTTC-ConA呈网格状分布,ECA小片状聚集分布.FITC-ConA的总体平均荧光强度较ECA高,差异有统计学意义(P＜0.001).结论 Uu体外培养生物膜主要呈网格状结构,胞外多糖中含有葡萄糖、甘露糖、半乳糖、N-乙酰葡聚糖残基,并以葡萄糖、甘露糖残基为主.
目的 研究解脲脲原體(Ureaplasma urealyticum,Uu)4箇型彆標準株在體外形成的生物膜之胞外多糖的分佈及結構成分.方法 將Uu標準株Parvo群中4、8血清型和T960群中3、14血清型進行體外生物膜培養後,掃描電鏡下觀察生物膜組成及結構,併在FITC-ConA/PI及ECA/PI雙熒光染色後進行激光共聚焦顯微鏡觀察及測定平均熒光彊度.秩和檢驗及t檢驗分彆比較兩種熒光標記物、兩生物群的總體平均熒光彊度的差異.結果 4箇型彆Uu標準株均可在體外形成生物膜,生物膜結構主要呈網格狀,胞外物質佔大部分比例.在激光共聚焦顯微鏡下,Uu生物膜胞外多糖均可被FITC-ConA和ECA染色,FTTC-ConA呈網格狀分佈,ECA小片狀聚集分佈.FITC-ConA的總體平均熒光彊度較ECA高,差異有統計學意義(P＜0.001).結論 Uu體外培養生物膜主要呈網格狀結構,胞外多糖中含有葡萄糖、甘露糖、半乳糖、N-乙酰葡聚糖殘基,併以葡萄糖、甘露糖殘基為主.
목적 연구해뇨뇨원체(Ureaplasma urealyticum,Uu)4개형별표준주재체외형성적생물막지포외다당적분포급결구성분.방법 장Uu표준주Parvo군중4、8혈청형화T960군중3、14혈청형진행체외생물막배양후,소묘전경하관찰생물막조성급결구,병재FITC-ConA/PI급ECA/PI쌍형광염색후진행격광공취초현미경관찰급측정평균형광강도.질화검험급t검험분별비교량충형광표기물、량생물군적총체평균형광강도적차이.결과 4개형별Uu표준주균가재체외형성생물막,생물막결구주요정망격상,포외물질점대부분비례.재격광공취초현미경하,Uu생물막포외다당균가피FITC-ConA화ECA염색,FTTC-ConA정망격상분포,ECA소편상취집분포.FITC-ConA적총체평균형광강도교ECA고,차이유통계학의의(P＜0.001).결론 Uu체외배양생물막주요정망격상결구,포외다당중함유포도당、감로당、반유당、N-을선포취당잔기,병이포도당、감로당잔기위주.
Objective To investigate the extracellular polysaccharide distribution and components of Ureaplasma urealyticum (Uu) after biofilm having been developed in.Methods The standard serotype 3 and serotype 14 belong to biovar Parvo,and the standard serotype 4 and serotype 8 belong to biovar T960 were employed to form biofilrns in vitro.Scanning electron microscope and confocal laser scanning microscope were used to analysis the biofilms and extracellular polysaccharide.We used combination of two different labeled lectins,Canavalia ensiformis(FITC-ConA) and Erythrina cristagalli(ECA) which bind to specific polysaccharide residues to visualize extracellular polysaccharide in biofilms,and average uorescence intensity was evaluated Results All the strains can form the biofilmsin vitro.The biofilm was honeycomb-Like structures mainly,and extracellular polymeric substances accounts for majority of proportions.All the extracellular polysaccharide could be combined with FITC-ConA and ECA,and the total average fluorescence intensity of FITC-ConA was higher than ECA( P＜0.001 ).Conclusion Ureaplasma urealyticum biofilm is honeycomb-like structures mainly.The extracellular polysaccharide contains,galactose,and N-acetyl glucan residual,and the glucose,mannose residual are the main components.
