眼科研究
眼科研究
안과연구
CHINESE OPHTHALMIC RESEARCH
2009年
11期
965-968
,共4页
廖华萍%张士胜%孙悦%王康孙%王玲
廖華萍%張士勝%孫悅%王康孫%王玲
료화평%장사성%손열%왕강손%왕령
激光%脉络膜新生血管%动物模型
激光%脈絡膜新生血管%動物模型
격광%맥락막신생혈관%동물모형
laser%choroidal neovascularization%animal model
目的 观察不同能量的半导体激光对诱导棕色挪威(BN)大鼠脉络膜新生血管(CNV)形成的影响.方法 雄性BN大鼠42只随机分组,将其中的36只根据激射激光的功率不同平均分为3个实验组,其余6只作为正常对照组.采用810nm半导体激光光凝大鼠双眼视网膜,光斑直径75μm,曝光时间0.1s,激光功率分别为120、140和160mW,于光凝后1、7、14、21、28、56d进行荧光素眼底血管造影(FFA)和吲哚菁绿血管造影(ICGA)检查,随后于每个时间点每组各处死2只大鼠,摘除眼球在光学显微镜下检查CNV形成情况.结果 不同能量半导体激光光凝BN大鼠视网膜后7dCNV开始形成,21d达高峰,此时根据FFA和ICGA计算的3组CNV的成模率分别为51.3%、91.8%、88.3%及51.3%、92.7%、93.7%,56d时CNV有所减少.光凝后7d可见光凝斑周围炎性细胞增多,CNV形成;14d光凝斑增厚,色素细胞增生、移行;21d时140mW及160mW组多数光凝斑增厚,可见明显CNV;随后炎性细胞减少,胶原纤维增多,56d时仍可见CNV结构.结论 功率分别为120、140、160mW的810nm半导体激光均能成功诱导BN大鼠形成CNV,140mW组成模率高且受干扰因素少,是建立BN大鼠CNV模型比较理想的参数.
目的 觀察不同能量的半導體激光對誘導棕色挪威(BN)大鼠脈絡膜新生血管(CNV)形成的影響.方法 雄性BN大鼠42隻隨機分組,將其中的36隻根據激射激光的功率不同平均分為3箇實驗組,其餘6隻作為正常對照組.採用810nm半導體激光光凝大鼠雙眼視網膜,光斑直徑75μm,曝光時間0.1s,激光功率分彆為120、140和160mW,于光凝後1、7、14、21、28、56d進行熒光素眼底血管造影(FFA)和吲哚菁綠血管造影(ICGA)檢查,隨後于每箇時間點每組各處死2隻大鼠,摘除眼毬在光學顯微鏡下檢查CNV形成情況.結果 不同能量半導體激光光凝BN大鼠視網膜後7dCNV開始形成,21d達高峰,此時根據FFA和ICGA計算的3組CNV的成模率分彆為51.3%、91.8%、88.3%及51.3%、92.7%、93.7%,56d時CNV有所減少.光凝後7d可見光凝斑週圍炎性細胞增多,CNV形成;14d光凝斑增厚,色素細胞增生、移行;21d時140mW及160mW組多數光凝斑增厚,可見明顯CNV;隨後炎性細胞減少,膠原纖維增多,56d時仍可見CNV結構.結論 功率分彆為120、140、160mW的810nm半導體激光均能成功誘導BN大鼠形成CNV,140mW組成模率高且受榦擾因素少,是建立BN大鼠CNV模型比較理想的參數.
목적 관찰불동능량적반도체격광대유도종색나위(BN)대서맥락막신생혈관(CNV)형성적영향.방법 웅성BN대서42지수궤분조,장기중적36지근거격사격광적공솔불동평균분위3개실험조,기여6지작위정상대조조.채용810nm반도체격광광응대서쌍안시망막,광반직경75μm,폭광시간0.1s,격광공솔분별위120、140화160mW,우광응후1、7、14、21、28、56d진행형광소안저혈관조영(FFA)화신타정록혈관조영(ICGA)검사,수후우매개시간점매조각처사2지대서,적제안구재광학현미경하검사CNV형성정황.결과 불동능량반도체격광광응BN대서시망막후7dCNV개시형성,21d체고봉,차시근거FFA화ICGA계산적3조CNV적성모솔분별위51.3%、91.8%、88.3%급51.3%、92.7%、93.7%,56d시CNV유소감소.광응후7d가견광응반주위염성세포증다,CNV형성;14d광응반증후,색소세포증생、이행;21d시140mW급160mW조다수광응반증후,가견명현CNV;수후염성세포감소,효원섬유증다,56d시잉가견CNV결구.결론 공솔분별위120、140、160mW적810nm반도체격광균능성공유도BN대서형성CNV,140mW조성모솔고차수간우인소소,시건립BN대서CNV모형비교이상적삼수.
Objective Choroidal neovascularization(CNV) is the main cause of blindness in over 50-years-old population. To establish an available CNV animal model is helpful for us to understand the pathogenesis and management of CNV. Purpose of present study was to observe the role of coagulation with different power of diode laser in laser-induced choroidal neovascularization model of Brown Norway (BN) rats. Methods Coagulation of 810 nm diode laser(8 - 10 spots for each eye) was performed in 36 male BN rats with the spot diameter 75 μm, shutter time 0. 1 s, power 120 mW, 140 mW and 160 mW, respectively, while 6 normal BN rats were used as contrast. CNV was evaluated by fundus examination, fundus fluorescein angiography (FFA), indocyanine green angiography(ICGA) and light microscope on day 1, 7, 14, 21, 28 and 56 after photocoagulation. Results CNV formed on the 7th day after photocoagulation in 120 mW, 140 mW and 160 mW groups and reached the peak on the 21st day according to FFA and ICGA manifestation. Incidence of CNV in 120 mW, 140 mW and 160 mW group was 51. 3%, 91. 8%, 88. 3% on FFA findings and 51.3%, 92.7%, 93.7% on ICGA findings, respectively. In 7 days after photocoagulation, inflammatory cells increased and CNV formed at the lesion. Photocoagulation plaque became thicker with pigment cells proliferating and migrating on 14 days. After that time, inflammatory cells decreased and more collagen fibers emerged. The CNV reminded till the 56th day after photocoagulation. Conclusion CNV model of BN rats can be successfully created using the different power of diode laser (from 120 through 160 mW). CNV rate under the laser coagulation with 140 mW is higher, indicating that the power of 140 mW may be a suitable parameter for diode laser-induced choroidal neovascularization model of BN rats.
