中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
1期
68-70
,共3页
Toll样受体4%动脉粥样硬化%单核细胞%小鼠,基因敲除
Toll樣受體4%動脈粥樣硬化%單覈細胞%小鼠,基因敲除
Toll양수체4%동맥죽양경화%단핵세포%소서,기인고제
Toll-like receptor 4%Atherosclerosis%Monoeytes%Mice,knockout
目的 通过观察替米沙坦对动脉粥样硬化(AS)模型小鼠单核细胞Toll样受体4(TLR4)表达的影响,探讨替米沙坦在AS治疗中的炎性机制. 方法 AS模型采用高脂餐喂养的ApoE基因缺陷小鼠.替米沙坦干预组在高脂餐喂养基础上给予替米沙坦.8周后,测定单纯高脂饮食组和替米沙坦干预组血清低密度脂蛋白、三酰甘油、总胆固醇水平,并应用流式细胞仪检测两组小鼠外周血单核细胞表面TLR4的表达. 结果 两组小鼠血脂水平无统计学差异.与单纯高脂饮食组相比,替米沙坦干预组小鼠TLR4表达明显降低(P＜0.05). 结论 替米沙坦可降低TLR4表达而干预其在AS进程中介导的炎性反应.
目的 通過觀察替米沙坦對動脈粥樣硬化(AS)模型小鼠單覈細胞Toll樣受體4(TLR4)錶達的影響,探討替米沙坦在AS治療中的炎性機製. 方法 AS模型採用高脂餐餵養的ApoE基因缺陷小鼠.替米沙坦榦預組在高脂餐餵養基礎上給予替米沙坦.8週後,測定單純高脂飲食組和替米沙坦榦預組血清低密度脂蛋白、三酰甘油、總膽固醇水平,併應用流式細胞儀檢測兩組小鼠外週血單覈細胞錶麵TLR4的錶達. 結果 兩組小鼠血脂水平無統計學差異.與單純高脂飲食組相比,替米沙坦榦預組小鼠TLR4錶達明顯降低(P＜0.05). 結論 替米沙坦可降低TLR4錶達而榦預其在AS進程中介導的炎性反應.
목적 통과관찰체미사탄대동맥죽양경화(AS)모형소서단핵세포Toll양수체4(TLR4)표체적영향,탐토체미사탄재AS치료중적염성궤제. 방법 AS모형채용고지찬위양적ApoE기인결함소서.체미사탄간예조재고지찬위양기출상급여체미사탄.8주후,측정단순고지음식조화체미사탄간예조혈청저밀도지단백、삼선감유、총담고순수평,병응용류식세포의검측량조소서외주혈단핵세포표면TLR4적표체. 결과 량조소서혈지수평무통계학차이.여단순고지음식조상비,체미사탄간예조소서TLR4표체명현강저(P＜0.05). 결론 체미사탄가강저TLR4표체이간예기재AS진정중개도적염성반응.
Objective To observe the effects of telmisartan on toll-like receptor (TLR) 4 expression in monocytes of mice atherosclerosis (AS) model, and explore the mechanism of inflammation. Methods AS mice model were established in ApoE-deficient mice fed with high-fat diet,and treated with telmisartan as intervention group. After 8 weeks, serum low density lipoprotein,triacylglycerol and total cholesterol were detected in group fed with high-fat diet and intervention group.TLR 4 expressions in two groups were analyzed by flow cytometry. Results There was no significant difference in levels of blood lipids between the two groups.TLR4 expression in monocytes in telmisartan intervention group was lower than group fed with high-fat diet (P＜0.05). Conclusions Telmisartan may intervene the inflammation during atherosclerosis by downregulating TLR4 expression in monocytes.
