中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
2期
103-106
,共4页
李海东%裘正军%黄陈%江弢%曹俊
李海東%裘正軍%黃陳%江弢%曹俊
리해동%구정군%황진%강도%조준
胰腺肿瘤%STAT3转录因子%肿瘤转移%基因
胰腺腫瘤%STAT3轉錄因子%腫瘤轉移%基因
이선종류%STAT3전록인자%종류전이%기인
Pancreatic neoplasms%STAT3 transcription-factor%Neoplasm metastasis%Genes
目的 应用基因芯片筛选信号转导与转录激活因子3(STAT3)调控胰腺癌侵袭转移的相关基因.方法 以慢病毒感染获得稳定STAT3沉默人胰腺癌细胞株SW1990,以空质粒病毒组、未感染组为对照.应用基因芯片筛选3组细胞与肿瘤侵袭转移相关的差异表达基因.用实时PCR及蛋白质印迹法检测STAT3 mRNA及蛋白表达,并验证所选取的3个差异表达基因(MMP-7、IL-1β、IgTα7).结果 靶向STAT3的病毒感染SW1990细胞后,STAT3 mRNA表达水平为0.391±0.037,显著低于空质粒病毒组的1.002±0.015和未感染组的1.206 ±0.042(P ＜0.05)；STAT3蛋白表达水平为182.38±65.32,亦明显低于空质粒病毒组的223.40±58.40和未感染组的212.33±53.69(P＜0.05).STAT3沉默的SW1990细胞有8个肿瘤侵袭转移相关基因表达上调,3个表达下调,经验证,沉默组细胞MMP-7、IL-1β mRNA表达水平明显低于空质粒病毒组(0.287±0.115比1.010±0.124,t =19.45,P=0.000；0.490±0.010比1.002±0.002,t=13.83,P=0.000),而IgTα7 mRNA表达水平无明显变化(1.173±0.280比0.998±0.003,t=4.236,P=0.094).同时沉默组细胞MMP-7蛋白表达亦显著降低.结论 STAT3沉默可导致人胰腺癌SW1990细胞多个与肿瘤侵袭转移相关基因表达的改变,其中MMP-7可能是受STAT3调控的主要靶基因.
目的 應用基因芯片篩選信號轉導與轉錄激活因子3(STAT3)調控胰腺癌侵襲轉移的相關基因.方法 以慢病毒感染穫得穩定STAT3沉默人胰腺癌細胞株SW1990,以空質粒病毒組、未感染組為對照.應用基因芯片篩選3組細胞與腫瘤侵襲轉移相關的差異錶達基因.用實時PCR及蛋白質印跡法檢測STAT3 mRNA及蛋白錶達,併驗證所選取的3箇差異錶達基因(MMP-7、IL-1β、IgTα7).結果 靶嚮STAT3的病毒感染SW1990細胞後,STAT3 mRNA錶達水平為0.391±0.037,顯著低于空質粒病毒組的1.002±0.015和未感染組的1.206 ±0.042(P ＜0.05)；STAT3蛋白錶達水平為182.38±65.32,亦明顯低于空質粒病毒組的223.40±58.40和未感染組的212.33±53.69(P＜0.05).STAT3沉默的SW1990細胞有8箇腫瘤侵襲轉移相關基因錶達上調,3箇錶達下調,經驗證,沉默組細胞MMP-7、IL-1β mRNA錶達水平明顯低于空質粒病毒組(0.287±0.115比1.010±0.124,t =19.45,P=0.000；0.490±0.010比1.002±0.002,t=13.83,P=0.000),而IgTα7 mRNA錶達水平無明顯變化(1.173±0.280比0.998±0.003,t=4.236,P=0.094).同時沉默組細胞MMP-7蛋白錶達亦顯著降低.結論 STAT3沉默可導緻人胰腺癌SW1990細胞多箇與腫瘤侵襲轉移相關基因錶達的改變,其中MMP-7可能是受STAT3調控的主要靶基因.
목적 응용기인심편사선신호전도여전록격활인자3(STAT3)조공이선암침습전이적상관기인.방법 이만병독감염획득은정STAT3침묵인이선암세포주SW1990,이공질립병독조、미감염조위대조.응용기인심편사선3조세포여종류침습전이상관적차이표체기인.용실시PCR급단백질인적법검측STAT3 mRNA급단백표체,병험증소선취적3개차이표체기인(MMP-7、IL-1β、IgTα7).결과 파향STAT3적병독감염SW1990세포후,STAT3 mRNA표체수평위0.391±0.037,현저저우공질립병독조적1.002±0.015화미감염조적1.206 ±0.042(P ＜0.05)；STAT3단백표체수평위182.38±65.32,역명현저우공질립병독조적223.40±58.40화미감염조적212.33±53.69(P＜0.05).STAT3침묵적SW1990세포유8개종류침습전이상관기인표체상조,3개표체하조,경험증,침묵조세포MMP-7、IL-1β mRNA표체수평명현저우공질립병독조(0.287±0.115비1.010±0.124,t =19.45,P=0.000；0.490±0.010비1.002±0.002,t=13.83,P=0.000),이IgTα7 mRNA표체수평무명현변화(1.173±0.280비0.998±0.003,t=4.236,P=0.094).동시침묵조세포MMP-7단백표체역현저강저.결론 STAT3침묵가도치인이선암SW1990세포다개여종류침습전이상관기인표체적개변,기중MMP-7가능시수STAT3조공적주요파기인.
Objective To screen the genes related with signal transducers and activators of transcription 3 (STAT3) regulating pancreatic cancer invasion and metastasis by gene chips. Methods Human pancreatic cancer cell line SW1990 stably expressing low level of Stat3 was established by lentivirus transfection,while cells transfected with mock plasmid and cells without transfection served as control groups.The differences of invasion and metastasis related genes expression among the three groups were screened by gene chips.STAT3 mRNA and protein expression was measured by real-time PCR and Western blot.Three differentially expressed genes (MMP-7,IL-1β and IgTα7) were verified.Results The expression level of STAT3 mRNA was 0.391 ± 0.037 after pancreatic cancer SW1990 cell trarsfected with STAT3 targeted lentivirus,which was significantly lower than those in mock plasmid group (1.002 ± 0.015) and nontransfected group ( 1.206 ± 0.042,P ＜ 0.05 ) ; the expression level of STAT3 protein was 182.38 ± 65.32,which was significantly lower than those in mock plasmid group (223.40 ±58.40) and non-transfected group (212.33 ±53.69).Eight invasion and metastasis related genes of SW1990 lowly expressing Stat3 were upregulated,while 3 genes were down-regulated.By verification,the mRNA level of MMP-7 and IL-1β were lower than in control group transfected with mook plassmid(0.287 ± 0.115 vs 1.010 ± 0.124,t =19.45,P =0.000;0.490 ± 0.10 vs 1.002 ± 0.002,t =13.83,P =0.000),but the mRNA level of IgTα7 was not decreased (1.173 ±0.280 vs 0.998 ±0.003,t =4.236,P =0.094).Meanwhile,the protein level of MMP-7 was significantly down-regulated when Stat3 was knocked down.Conclusions Stat3 causes changes of expressions of many invasion and metastasis-related genes of SW1990,and MMP-7 may be the main target gene regulated by Stat3.
