CAJ | 학술논문

目的探讨不同水平循环牵张应力对人退变椎间盘细胞增殖的影响.方法取1例29岁手术切除的腰椎间盘突出患者的椎间盘,通过病理学方法评价其退变程度,酶消化法分离椎间盘纤维环细胞.选取P3代细胞,利用ElectroForce 3200力学试验仪搭载的BioDynamic生物反应舱系统,以3h为固定时间点对其进行不同水平的频率为0.25 Hz的周期性牵张应力刺激,设置形变量分别为0、5％、10％、15％和20％,应用流式细胞术检测不同应力刺激下纤维环细胞的增殖率变化情况,观察并探讨不同循环牵张应力对体外培养的人体退变椎间盘细胞的影响.结果随着牵张应力的增加,纤维环细胞S期细胞比例和增殖指数(PI)呈缓慢上升趋势,15％时达到峰值,之后开始下降.结论体外培养的人退变椎间盘纤维环细胞的增殖活性与受力大小有关且存在细胞增殖的最适应力条件；超过此应力范围,其增殖活性将会降低.
목적 탐토불동수평순배견장응력대인퇴변추간반세포증식적영향.방법 취1례29세수술절제적요추간반돌출환자적추간반,통과병이학방법평개기퇴변정도,매소화법분리추간반섬유배세포.선취P3대세포,이용ElectroForce 3200역학시험의탑재적BioDynamic생물반응창계통,이3h위고정시간점대기진행불동수평적빈솔위0.25 Hz적주기성견장응력자격,설치형변량분별위0、5％、10％、15％화20％,응용류식세포술검측불동응력자격하섬유배세포적증식솔변화정황,관찰병탐토불동순배견장응력대체외배양적인체퇴변추간반세포적영향.결과 수착견장응력적증가,섬유배세포S기세포비례화증식지수(PI)정완만상승추세,15％시체도봉치,지후개시하강.결론 체외배양적인퇴변추간반섬유배세포적증식활성여수력대소유관차존재세포증식적최괄응력조건；초과차응력범위,기증식활성장회강저.
Objective To investigate the effects of different cyclic tensile strain on the proliferation of human anulus fibrosus cells from degenerated discs.Methods Anulus fibrosus(AF) cells were isolated from a degenerated human IVD,expanded in monolayer,and cyclically strained for 3 hours,applying 0,5％,10％,15％ and 20％ strains at a frequency of 0.25 Hz with the use of the DioDynamic test instrument.The flow cytometry method was used to examine the Af cells proliferation at 24 hours following application of the cyclic tensile strains.Results The proliferative index (PI) increased with the magnitude value of cyclic tensile strain except 20％ group.The most significant increase of proliferation index were found in 15％ group.Conclusion There might be some corelationships between magnitude of cyclic tensile strain and the proliferation of the degenerative AF cells.