植物研究
植物研究
식물연구
BULLETIN OF BOTANICAL RESEARCH
2008年
6期
720-725
,共6页
吴茱萸%扩增片段长度多态性%指纹图谱%聚类分析
吳茱萸%擴增片段長度多態性%指紋圖譜%聚類分析
오수유%확증편단장도다태성%지문도보%취류분석
Evodia rutaecarpa (Juss.) Benth%Amplified fragment length polymorphism%DNA finger-print%Clustering analysis
首次报道中草药植物吴茱萸基因组DNA指纹图谱的研究.吴茱萸是贵州省内经济价值极高的中草药之一,采用扩增片段长度多态性(AFLP)技术来分析来自不同地区的石虎、疏毛、大花吴茱萸3个品种的DNA指纹图谱,从18对引物中筛选出3对引物对19份材料的DNA检测,共得到93条带,其中多态性片段57条(平均61.3%).3对引物组合从DNA指纹图谱上将19份材料完全区分开,结果表明AFLP技术是鉴别吴茱萸相近品种的有效方法,是形态学鉴定方法的有益补充;UPGMA方法聚类分析显示19份种质材料间的相似系数为0.235～0.941,表明昊茱萸种质间的遗传多样性丰富;余庆地区种植基地的石虎和疏毛样本聚为一类,提示人工栽培影响到吴茱萸的遗传特性.
首次報道中草藥植物吳茱萸基因組DNA指紋圖譜的研究.吳茱萸是貴州省內經濟價值極高的中草藥之一,採用擴增片段長度多態性(AFLP)技術來分析來自不同地區的石虎、疏毛、大花吳茱萸3箇品種的DNA指紋圖譜,從18對引物中篩選齣3對引物對19份材料的DNA檢測,共得到93條帶,其中多態性片段57條(平均61.3%).3對引物組閤從DNA指紋圖譜上將19份材料完全區分開,結果錶明AFLP技術是鑒彆吳茱萸相近品種的有效方法,是形態學鑒定方法的有益補充;UPGMA方法聚類分析顯示19份種質材料間的相似繫數為0.235～0.941,錶明昊茱萸種質間的遺傳多樣性豐富;餘慶地區種植基地的石虎和疏毛樣本聚為一類,提示人工栽培影響到吳茱萸的遺傳特性.
수차보도중초약식물오수유기인조DNA지문도보적연구.오수유시귀주성내경제개치겁고적중초약지일,채용확증편단장도다태성(AFLP)기술래분석래자불동지구적석호、소모、대화오수유3개품충적DNA지문도보,종18대인물중사선출3대인물대19빈재료적DNA검측,공득도93조대,기중다태성편단57조(평균61.3%).3대인물조합종DNA지문도보상장19빈재료완전구분개,결과표명AFLP기술시감별오수유상근품충적유효방법,시형태학감정방법적유익보충;UPGMA방법취류분석현시19빈충질재료간적상사계수위0.235～0.941,표명호수유충질간적유전다양성봉부;여경지구충식기지적석호화소모양본취위일류,제시인공재배영향도오수유적유전특성.
In the present study,the DNA fingerprints of Fructus Evodiae was reported. Fructus Evodiae is one of the most commercially important herbs in Guizhou Province. Amplified fragment length polymor-phism(AFLP) techniques was used to analyze the DNA fingerprints of 3 types of Fructus Evodiae inclu-ding Evodia rutaecarpa var. officinalis , E. rutaecarpa var. bodinieri and E. rutaecarpa (Juss.) Benth. Three primer combinations (E-AGC/M-CAG, E-AGC/M-CTG and E-ACG/M-CTG) screened out from eighteen primer pairs were used to conduct genetic analysis and variety identification of nineteen materi-als of Fructus Evodiae. 93 bands were generated and 57 fragments appeared to be polymorphic(61.3% on average). The fragment profiles of the nineteen materials were obtained and could be distinguished from each other. The results showed that AFLP was an efficient method to identify the germplasms of Fructus Evodiae and could be a supplement for the morphological identifcation methods for Fructus Evo-diae. UPGMA cluster analysis was also performed. Genetic similarity coefficient(SC) ranged from 0.235 to 0.941 ,indicating that Fructus Evodiae is rich in genetic diversity. Dendrogram revealed that E. ru-taecarpa vat. officinalis and E. rutaecarpa var. bodinieri from Yuqing region were clustered into one group ,which suggested that human cultivation activities have influenced on the genetic characteristics of Fructus Evodiae.
