CAJ | 학술논문

目的：探讨不同浓度的10-HDA对体外培养大鼠小脑神经细胞的增殖和存活的影响。方法：将不同浓度的10-HDA加到原代培养的新生大鼠小脑神经细胞中,使其终浓度分别为0.1μmol/L、1.0μmol/L、10μmol/L、通过BrdU染色,尼氏染色,MTT的方法来检测10-HDA对体外培养大鼠小脑神经细胞增殖和存活的影响。结果：不同浓度的10-HDA均能促进小脑细胞的增殖和存活,并且有一定的浓度依赖关系。中浓度组（10-HDA终浓度为1.0μmol/L）的效果最为显著,与对照组相比有显著性差异（p〈0.01）。结论：10-HDA有助于小脑神经细胞的增殖和存活。
목적：탐토불동농도적10-HDA대체외배양대서소뇌신경세포적증식화존활적영향。방법：장불동농도적10-HDA가도원대배양적신생대서소뇌신경세포중,사기종농도분별위0.1μmol/L、1.0μmol/L、10μmol/L、통과BrdU염색,니씨염색,MTT적방법래검측10-HDA대체외배양대서소뇌신경세포증식화존활적영향。결과：불동농도적10-HDA균능촉진소뇌세포적증식화존활,병차유일정적농도의뢰관계。중농도조（10-HDA종농도위1.0μmol/L）적효과최위현저,여대조조상비유현저성차이（p〈0.01）。결론：10-HDA유조우소뇌신경세포적증식화존활。
【Objective】To study the effects of 10-HDA on the proliferation and survival of cultured neurons from cerebellum of newborn rats.【Method】10-HDA of different concentration were added to the cultured cells（final concentration were 0.1μmol/L,1.0μmol/L,and 10μmol/L respectively）.The proliferation and development of cerebellar nerves were detected using the technique of BrdU staining,nissl staining,thiazolyl blue colorimetry（MTT）.【Result】10-HDA advanced the proliferation and survival of cerebellar nerve cells.The effect of 10-HDA with the final concentration of 1.0μmol/L was the most significant.【Conclusion】10-HDA could advance the proliferation and survival of cerebellar nerve cells.