CAJ | 학술논문

本研究根据Genbank登录的猪传染性胃肠炎病毒（TGEv）N基因和S基因保守区序列设计合成了两对引物和探针，通过对荧光定量RT-PCR反应条件的优化，建立了TaqMan荧光定量RT-PCR快速检测TGEV的方法。该方法能有效地鉴别序列密切相关的猪传染性胃肠炎病毒与呼吸道冠状病毒。与常规RT-PCR试验比较表明，所建立的荧光RT-PCR检测技术快速、敏感，检测时限3个小时以内，具有很好的特异性和重复性。通过对39份临床样品进行检测，结果表明所建立的检测方法直接检测样品中猪传染性胃肠炎病毒。
본연구근거Genbank등록적저전염성위장염병독（TGEv）N기인화S기인보수구서렬설계합성료량대인물화탐침，통과대형광정량RT-PCR반응조건적우화，건립료TaqMan형광정량RT-PCR쾌속검측TGEV적방법。해방법능유효지감별서렬밀절상관적저전염성위장염병독여호흡도관상병독。여상규RT-PCR시험비교표명，소건립적형광RT-PCR검측기술쾌속、민감，검측시한3개소시이내，구유흔호적특이성화중복성。통과대39빈림상양품진행검측，결과표명소건립적검측방법직접검측양품중저전염성위장염병독。
The probes and primers were designed and synthesized based on the conserved gene N and S of transmissible gastroenteritis virus （TGEV） available in GenBank, and the reaction parameters were optimized to develop a sensitive TaqMan-based real-time RT-PCR assay for the rapid detection of TGEV. The assay could differentiate TGEV from porcine respiratory coronavirus （PRCV） effectively. Compared with normal RT-PCR, the developed RT-PCR was fast and sensitive, with the detection time less than 3 hours. And the specificity and reproducibility of the assay were very good. By detecting 39 clinical samples, the results suggested that the method could be used to detect TGEV in samples effectively.