吉林体育学院学报
吉林體育學院學報
길림체육학원학보
JOURNAL OF JILIN INSTITUTE OF PHYSICAL EDUCATION
2011年
6期
1-4
,共4页
仇乃民%郭成吉%赵光高%马思远
仇迺民%郭成吉%趙光高%馬思遠
구내민%곽성길%조광고%마사원
有氧运动%热应激%白细胞介素-2%超氧化物歧化酶
有氧運動%熱應激%白細胞介素-2%超氧化物歧化酶
유양운동%열응격%백세포개소-2%초양화물기화매
aerobic exercise%heat stress%interleukin - 2%SOD
目的：探讨有氧训练对机体运动热应激下免疫机能与自由基代谢的影响及其可能机制。方法：健康雄性sD大鼠51只，随机选取45只分为对照组（A组）、热应激组（B组）、6周有氧训练组（c组）、1次有氧运动加热应激组（D组）与6周有氧训练末次加热应激组（E组），其余6只用-P＊L酸阈强度的测定。各组实验后取血清测定IL-2、SOD、MDA水平。结果：（1）B、D、E组经高温暴露后，大鼠肛温迅速升至39．65±0．250C、41．02±0．140C与39．97±0．290C，均表现出呼吸急促、流汗、烦躁不安等，为典型的热应激症候。（2）B组血清IL-2、SOD、SOD／MDA均显著低于A组，MDA高于A组；D组血清IL-2、SOD、SOD／MDA均显著低于A组（P〈0．01）与B组（P〈0．05），MDA显著高于A组（P〈0．01）与B组（P〈0．05）。（3）c组血清IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）高于A组，MDA显著低于A组；E组血清IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）均高于D组，MDA显著低于D组。结论：运动热应激可导致自由基生成增多，加重组织细胞膜结构与功能的损害，导致温度调节的信号通路中断，体温失衡，可能通过调节神经-内分泌-免疫系统，抑制IL-2的分泌。IL-2可作为评价机体运动热应激下免疫机能变化的重要指标。有氧训练可拮抗运动热应激对免疫系统的损害。
目的：探討有氧訓練對機體運動熱應激下免疫機能與自由基代謝的影響及其可能機製。方法：健康雄性sD大鼠51隻，隨機選取45隻分為對照組（A組）、熱應激組（B組）、6週有氧訓練組（c組）、1次有氧運動加熱應激組（D組）與6週有氧訓練末次加熱應激組（E組），其餘6隻用-P＊L痠閾彊度的測定。各組實驗後取血清測定IL-2、SOD、MDA水平。結果：（1）B、D、E組經高溫暴露後，大鼠肛溫迅速升至39．65±0．250C、41．02±0．140C與39．97±0．290C，均錶現齣呼吸急促、流汗、煩躁不安等，為典型的熱應激癥候。（2）B組血清IL-2、SOD、SOD／MDA均顯著低于A組，MDA高于A組；D組血清IL-2、SOD、SOD／MDA均顯著低于A組（P〈0．01）與B組（P〈0．05），MDA顯著高于A組（P〈0．01）與B組（P〈0．05）。（3）c組血清IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）高于A組，MDA顯著低于A組；E組血清IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）均高于D組，MDA顯著低于D組。結論：運動熱應激可導緻自由基生成增多，加重組織細胞膜結構與功能的損害，導緻溫度調節的信號通路中斷，體溫失衡，可能通過調節神經-內分泌-免疫繫統，抑製IL-2的分泌。IL-2可作為評價機體運動熱應激下免疫機能變化的重要指標。有氧訓練可拮抗運動熱應激對免疫繫統的損害。
목적：탐토유양훈련대궤체운동열응격하면역궤능여자유기대사적영향급기가능궤제。방법：건강웅성sD대서51지，수궤선취45지분위대조조（A조）、열응격조（B조）、6주유양훈련조（c조）、1차유양운동가열응격조（D조）여6주유양훈련말차가열응격조（E조），기여6지용-P＊L산역강도적측정。각조실험후취혈청측정IL-2、SOD、MDA수평。결과：（1）B、D、E조경고온폭로후，대서항온신속승지39．65±0．250C、41．02±0．140C여39．97±0．290C，균표현출호흡급촉、류한、번조불안등，위전형적열응격증후。（2）B조혈청IL-2、SOD、SOD／MDA균현저저우A조，MDA고우A조；D조혈청IL-2、SOD、SOD／MDA균현저저우A조（P〈0．01）여B조（P〈0．05），MDA현저고우A조（P〈0．01）여B조（P〈0．05）。（3）c조혈청IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）고우A조，MDA현저저우A조；E조혈청IL-2、SOD（P〈0．05）、SOD／MDA（P〈0．05）균고우D조，MDA현저저우D조。결론：운동열응격가도치자유기생성증다，가중조직세포막결구여공능적손해，도치온도조절적신호통로중단，체온실형，가능통과조절신경-내분비-면역계통，억제IL-2적분비。IL-2가작위평개궤체운동열응격하면역궤능변화적중요지표。유양훈련가길항운동열응격대면역계통적손해。
Objective： To investigate influences of aerobic exercise training on body immune function and free radical metabolism under heat stress and its pos- sible mechanism. Mcthods： 45 of 51 healthy male SD rats were randomly selected and divided into control group （group A）,heat stress group （group B）,6 weeks of aerobic training group （group C） ,a bout of aerobic exercise and heat stress group （group D） and 6 weeks of aerobic training and heat stress group （ group E）, and the remaining six for the determination of lactate threshold intensity. IL - 2, SOD, MDA levels of each group were measured in serum obtained after experiment. Results： （ 1 ） after high temperature exposure, in group B, D, E rat rectal temperature rapidly to 39.65 ± 0.25 ℃,41.02 ± 0.14℃ and 39. 97± 0.29℃ respectively ,with rapid breathing, sweating, irritability, etc., which are typical symptoms of heat stress. （2） levels of serum IL- 2, SOD, SOD/MDA in group B were significantly lower than that in group A,MDA was higher than that in group A; D senan levels of IL- 2,SOD,SOD/MDA were significantly lower than that in group A （ P 〈 0.01 ） and gronp B （ P 〈 0.05）, MDA was significantly higher than that in group A （ P 〈 0.01 ） and group B （ P 〈 0.05 ）. （3） In group C, serum levds of IL - 2, SOD （ P 〈 0.05）, SOD/MDA （ P 〈 0.05 ） were higher than that in group A, MDA was significantly lower than that in group A; In group E, serum levels of IL - 2, SOD （ P 〈0. 05 ）, SOD/MDA （ P 〈 0.05） were higher than that in group D, MDA was sig- nificantly Lower than that in group D. Conclusion： Exercise heat stress may cause mare free radicals, and increase damage of cell membrane structure and function of tisme, leading to interruption of the signaling pathways re4gulating the temperature, and imbalance of temperature, Possibly through regulation of nerve - endocrine - immune function and inhibition of IL - 2 secretion. IL - 2 can be used as an inmportant indicator of immune function changes after body movement under heat stress. Aerobic exercise training could reverse damage of immune system by heat stress.
