CAJ | 학술논문

本文利用生物信息学软件分析了鲤（Cyprinus carpio）基因组中同源框基因Hoxa3a与多种鱼类及其它生物的同源性,构建了分子进化树。结果表明：鲤与斑马鱼（Danio rerio）的序列同源性最高为95%,与大西洋鲑（Salmo salar）等四种鱼类的同源性次之为84%,与米氏叶吻银鲛（Callorhinchus milii）和鳐（Leucoraja erinacea）的同源性最低为74.5%,与三种鸟类的同源性为75%左右。这结果也说明,Hox同源框基因序列在进化上高度保守。在序列比较的同时发现了2个鲤的SNP位点,T101C和A213G。上述研究结果,对于保护生物多样性（尤其是遗传多样性）的研究,揭示生物进化历程及机理具有十分重要的意义。
본문이용생물신식학연건분석료리（Cyprinus carpio）기인조중동원광기인Hoxa3a여다충어류급기타생물적동원성,구건료분자진화수。결과표명：리여반마어（Danio rerio）적서렬동원성최고위95%,여대서양해（Salmo salar）등사충어류적동원성차지위84%,여미씨협문은교（Callorhinchus milii）화요（Leucoraja erinacea）적동원성최저위74.5%,여삼충조류적동원성위75%좌우。저결과야설명,Hox동원광기인서렬재진화상고도보수。재서렬비교적동시발현료2개리적SNP위점,T101C화A213G。상술연구결과,대우보호생물다양성（우기시유전다양성）적연구,게시생물진화역정급궤리구유십분중요적의의。
The sequence homology and molecular evolution of Hoxa3a gene were analyzed in common carp and other fishes and organisms by some bioinformatics softs.The results showed that the maximal sequence homology（up to 95%） was observed in common carp（Cyprinus carpio） and zebrafish（Danio rerio）,followed by Atlantic salmon（Salmo salar）,Japanese pufferfish（Takifugu rubripes）,Japanese medaka（Oryzias latipes） and Cichlid fish（Astatotilapia burtoni）（about 84%）,and the minimal（74.5%） in other fishes such as lttle skate（Leucoraja erinacea） and Australian ghost shark（Callorhinchus milii）.The sequence homology of common carp with three non-fish organisms was found to be 75%.This result also showed that Hox gene sequences were highly conserved evolutionarily.In the sequence comparison at the same time,two common carp SNP sites 101 site（T/C） and 213 site（C/T） were found.The result has great significance for the conservation of biological diversity（particularly genetic diversity） study and reveals the progress and mechanism of biological evolution.