CAJ | 학술논문

研究了不同提取方法和不同生长时期甘蔗提取的DNA对转基因甘蔗植株PCR检测的影响。结果表明,幼苗时期提取的甘蔗叶片模板DNA质量高于田间成熟植株;甘蔗幼苗时期的PCR检测易于成熟植株;CTAB法、SDS法和CTAB-SDS法提取的甘蔗叶片DNA均能满足幼苗期甘蔗抗性植株的PCR检测要求;对成熟转基因甘蔗植株而言,以CTAB-SDS法提取的DNA进行PCR检测的效果最佳。
연구료불동제취방법화불동생장시기감자제취적DNA대전기인감자식주PCR검측적영향。결과표명,유묘시기제취적감자협편모판DNA질량고우전간성숙식주;감자유묘시기적PCR검측역우성숙식주;CTAB법、SDS법화CTAB-SDS법제취적감자협편DNA균능만족유묘기감자항성식주적PCR검측요구;대성숙전기인감자식주이언,이CTAB-SDS법제취적DNA진행PCR검측적효과최가。
The effects of three template DNA extraction methods from different growth stages on transgenic sugarcane PCR detection were compared. The results indicated that the quality of DNA extracting from seedling leaves was better than that of mature plants and mature stage, and PCR detections were easier; DNA extracted from sugarcane leaves with CTAB method, SDS method and CTAB-SDS method were all suitable for PCR detection of resistant plants in seedling stage. The quality of DNA extracted with CTAB-SDS method was the best for PCR detection of resistant plants in mature stage.