CAJ | 학술논문

目的探讨提高小鼠胰岛分离纯化后数量和质量的适宜消化条件。方法通过胆总管逆行灌注胶原酶P溶液进行小鼠胰腺消化，分离胰岛，采用不连续密度梯度Ficoll离心法纯化胰岛，观察胶原酶浓度和消化时间对胰岛分离结果的影响。结果胶原酶浓度和消化时间对小鼠胰岛分离效果有重要影响。在胶原酶P浓度为1．0mg／ml、37℃消化25min条件下，胰岛分离效果最佳。结论胶原酶浓度和消化时间影响小鼠胰岛分离结果，优化消化条件可提高胰岛分离数量和质量。
목적탐토제고소서이도분리순화후수량화질량적괄의소화조건。방법통과담총관역행관주효원매P용액진행소서이선소화，분리이도，채용불련속밀도제도Ficoll리심법순화이도，관찰효원매농도화소화시간대이도분리결과적영향。결과효원매농도화소화시간대소서이도분리효과유중요영향。재효원매P농도위1．0mg／ml、37℃소화25min조건하，이도분리효과최가。결론효원매농도화소화시간영향소서이도분리결과，우화소화조건가제고이도분리수량화질량。
Objective To evaluate which digestive conditions could improve the yield of mouse pancreatic islets isolation. Methods Dif- ferent concentration of collagenase P was used by pancreatic intraduetal injection, and the pancreas was digested for different times. After digestion, the islets were purified using diseontinuous gradient centrifugation. The number, purity, and viability of the isolated islets were evalu- ated. Results The concentration of eollagenase P and the time of digestion were important factors for efficacious isolation of islets.The condition of digestion by 1.0 mg/ml collagenase P for 25 min was optimal for islet isolation. Conclusion The eollagenase Concentration and the digestion time were important factors for islet isolation. Optimizing conditions for pancreas digestion can improve islet purity and viability of mouse islet isolation.