中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2010年
9期
749-751
,共3页
常淑婷%毛志芹%易彪%尹晓波%阮强%何蓉%吉耀华%孙峥嵘%马艳萍%齐莹
常淑婷%毛誌芹%易彪%尹曉波%阮彊%何蓉%吉耀華%孫崢嶸%馬豔萍%齊瑩
상숙정%모지근%역표%윤효파%원강%하용%길요화%손쟁영%마염평%제형
人巨细胞病毒%UL146基因%多态性%趋化因子
人巨細胞病毒%UL146基因%多態性%趨化因子
인거세포병독%UL146기인%다태성%추화인자
Human cytomegalovims%UL146 gene%polymorphism%chemokine
目的研究人巨细胞病毒（HCMV）UL146基因在巨细胞病毒感染临床株中的多态性,寻找具有临床诊断、治疗价值的基因,为HCMV感染的临床诊断和防治提供资料。方法选择经QPCR检测尿HCMV-DNA为阳性的临床株进行UL146基因序列PCR扩增,对扩增阳性标本进行序列测定和分析。结果 28株扩增阳性标本序列之间有很大变异,种系树化分析结果显示可以分为3个型,4个亚型;ELRCXC的趋化因子功能区在各序列中高度保守。结论 HCMV-UL146基因呈现高度多态性,其编码的ELRCXC趋化因子功能区在各序列中高度保守,HCMV-UL146基因多态性与HCMV感染不同临床症状之间的关系尚不明确。
目的研究人巨細胞病毒（HCMV）UL146基因在巨細胞病毒感染臨床株中的多態性,尋找具有臨床診斷、治療價值的基因,為HCMV感染的臨床診斷和防治提供資料。方法選擇經QPCR檢測尿HCMV-DNA為暘性的臨床株進行UL146基因序列PCR擴增,對擴增暘性標本進行序列測定和分析。結果 28株擴增暘性標本序列之間有很大變異,種繫樹化分析結果顯示可以分為3箇型,4箇亞型;ELRCXC的趨化因子功能區在各序列中高度保守。結論 HCMV-UL146基因呈現高度多態性,其編碼的ELRCXC趨化因子功能區在各序列中高度保守,HCMV-UL146基因多態性與HCMV感染不同臨床癥狀之間的關繫尚不明確。
목적연구인거세포병독（HCMV）UL146기인재거세포병독감염림상주중적다태성,심조구유림상진단、치료개치적기인,위HCMV감염적림상진단화방치제공자료。방법선택경QPCR검측뇨HCMV-DNA위양성적림상주진행UL146기인서렬PCR확증,대확증양성표본진행서렬측정화분석。결과 28주확증양성표본서렬지간유흔대변이,충계수화분석결과현시가이분위3개형,4개아형;ELRCXC적추화인자공능구재각서렬중고도보수。결론 HCMV-UL146기인정현고도다태성,기편마적ELRCXC추화인자공능구재각서렬중고도보수,HCMV-UL146기인다태성여HCMV감염불동림상증상지간적관계상불명학。
Objective To investigate the polymorphism of human cytomegalovirus（HCMV）UL146 gene in clinical strains,and to evaluate its clinical diagnostic and therapeutic value of gene.Methods The UL146 gene of clinical strains was examined by quantitative polymerase chain reaction（Q-PCR）or general polymerase chain reaction（PCR）.Positive samples of PCR amplification were sequenced and analyzed.Results High variability of UL146 gene was found among 28 HCMV strains.According to phylogenetic analysis,all sequences of UL146 in clinical strains could be divided into three types and four subtypes.Chemokine ELRCXC region was highly conserved in all sequences.Conclusion HCMV-UL146 genes showed a high degree of polymorphism,and its encoded chemokine ELRCXC region was highly con-served.The relationship between HCMV-UL146 gene′s polymorphism and different clinical symptoms of HCMV infection was unclear.
