CAJ | 학술논문

采用纤维素酶和超声波辅助提取葡萄籽油,选择最佳工艺,并对葡萄籽油的提取率和理化性质进行比较研究.结果表明,纤维素酶辅助提取的最佳工艺参数:酶解温度50℃、酶解时间1.5h、酶解pH值为5.5、酶用量300U/g葡萄籽;超声波辅助提取的最佳工艺参数:超声功率500W,超声温度30℃,超声时间30min,料液比1:12(g/ml).超声波法的提油率高于纤维素酶辅助提取法,且时间较短、成本较低;而从葡萄籽油的品质上看,纤维素酶辅助提取的葡萄籽油相对密度与超声波法相当,酸价、过氧化值、碘值和水分及挥发物含量均低于超声波辅助法,折光指数高于超声波法.
채용섬유소매화초성파보조제취포도자유,선택최가공예,병대포도자유적제취솔화이화성질진행비교연구.결과표명,섬유소매보조제취적최가공예삼수:매해온도50℃、매해시간1.5h、매해pH치위5.5、매용량300U/g포도자;초성파보조제취적최가공예삼수:초성공솔500W,초성온도30℃,초성시간30min,료액비1:12(g/ml).초성파법적제유솔고우섬유소매보조제취법,차시간교단、성본교저;이종포도자유적품질상간,섬유소매보조제취적포도자유상대밀도여초성파법상당,산개、과양화치、전치화수분급휘발물함량균저우초성파보조법,절광지수고우초성파법.
In the present study, grape seed oil extraction technique with the assistance of cellulase or ultrasonic was optimized using orthogonal array design. In addition to this, grape seed oil samples obtained using both techniques were also evaluated for physico-chemical properties. Results showed that the optimum values of technological parameters were hydrolysis tempera-ture 50 ℃, hydrolysis time 1.5 h, pH 5.5, and enzyme dose 300 U/g for cellulase-assisted extraction, and ultrasound power 500 W, temperature 30 ℃, treatment 30 min and solid/liquid ratio 1:12 (g/ml) for ultrasonic-assisted extraction, respectively. Ultrasonic-assisted extraction gave higher oil yield and had the advantages of time saving and low cost when compared with cellulase-assisted extraction. However, lower peroxidation, acid and iodine values and moisture and volatile matter contents, higher refractive index and slightly different relative density were observed in grape seed oil obtained using cellulase-assisted extraction in comparison with using ultrasonic-assisted extraction. Therefore, from the viewpoint of product quality, cellulase-assisted extraction is superior to ultrasonic-assisted extraction.