CAJ | 학술논문

目的:观察隔药灸、温和灸、电针对克罗恩病(Crohn's Disease,CD)大鼠结肠碱性成纤维细胞生长因子(Basic Fibroblast Growth Factor,bFGF)、Ⅰ型胶原,(Collagen Type Ⅰ,Coll)表达的调控作用,探讨针灸治疗克罗恩病肠壁纤维化的作用机制.方法:采用TNBS多次致炎方法制备大鼠克罗恩病模型.将大鼠随机分为正常组、模型组、温和灸组、电针组和隔药灸组.各针灸治疗组选取天枢、气海穴分别采用不同的针灸方法进行治疗.采用免疫组织化学法观察大鼠结肠组织Coll、bFGF蛋白的分布和表达.结果:模型组大鼠结肠组织Col Ⅰ、bFGF蛋白表达显著增强(与正常组相比,P<0.01);经过隔药灸、温和灸、电针治疗后,CD大鼠Col Ⅰ、bFGF蛋白表达明显减少(P<0.01).结肠组织bFGF与Col Ⅰ蛋白Spearman等级相关分析结果表明,两者具有明显相关性(P<0.01).结论:针灸可以下调CD大鼠结肠异常增高的Col Ⅰ、bFGF蛋白表达.bFGF、Col Ⅰ蛋白参与了大鼠CD肠纤维化过程,针灸可能通过降低结肠bFGF蛋白表达,从而调节Col Ⅰ蛋白的沉积而发挥对CD肠纤维化的治疗作用.
목적:관찰격약구、온화구、전침대극라은병(Crohn's Disease,CD)대서결장감성성섬유세포생장인자(Basic Fibroblast Growth Factor,bFGF)、Ⅰ형효원,(Collagen Type Ⅰ,Coll)표체적조공작용,탐토침구치료극라은병장벽섬유화적작용궤제.방법:채용TNBS다차치염방법제비대서극라은병모형.장대서수궤분위정상조、모형조、온화구조、전침조화격약구조.각침구치료조선취천추、기해혈분별채용불동적침구방법진행치료.채용면역조직화학법관찰대서결장조직Coll、bFGF단백적분포화표체.결과:모형조대서결장조직Col Ⅰ、bFGF단백표체현저증강(여정상조상비,P<0.01);경과격약구、온화구、전침치료후,CD대서Col Ⅰ、bFGF단백표체명현감소(P<0.01).결장조직bFGF여Col Ⅰ단백Spearman등급상관분석결과표명,량자구유명현상관성(P<0.01).결론:침구가이하조CD대서결장이상증고적Col Ⅰ、bFGF단백표체.bFGF、Col Ⅰ단백삼여료대서CD장섬유화과정,침구가능통과강저결장bFGF단백표체,종이조절Col Ⅰ단백적침적이발휘대CD장섬유화적치료작용.
Objective:To observe the impacts of herb-partitioned moxibustion,warm moxibustion and electroacupuncture on the basic fibroblast growth factor(bFGF)and collagen type Ⅰ(Col Ⅰ)in colons of rats with Crohn' disease(CD),and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.Methods:The model rats were developed by TNBS as multiple proinflammatory method.The rats were randomly divided into 5 groups:a normal group,a model group,a warm moxibustion group,an electroacupuncture group and a herb-partitioned moxibustion group.The treatments were carried out at Tianshu(ST 25)(bilateral)and Qihai(CV 6)in different treatments.The immunohistochemistry was used to detect the expression position of Col Ⅰ and bFGF.Results:The expressions of Col Ⅰ and bFGF in colons of rots in the model group significantly increased(compared with the normal group,P<0.01).After the herb-partitioned moxibustion,warm moxibustion and electroacupuncture,the expressions of Col Ⅰ and bFGF reduced markedly in the rats with CD(P<0.01).The expression of bFGF and Col Ⅰ in the colons had an obvious correlation in the Spearman rank correlation analysis.Conclusion:Acupuncture treatment reduced the abnormally high levels of expressions for Col Ⅰ and bFGF in colons.Col Ⅰ and bFGF participated in the fibrosis.Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition,treating the intestinal fibrosis in CD.