CAJ | 학술논문

[目的]探索经不同菌源诱导黄粉虫所产生的免疫物质--抗菌肽对病原菌是否存在活性差异.[方法]对饥饿后黄粉虫幼虫通过饲细菌、针刺饲真菌(放线菌)法产生诱导免疫反应,研磨、冷冻离心法获得抗菌肽提取物,采用抑菌圈法测定其对26种病原菌抑菌活性,利用三等分法划分四种类型评价抑菌活性及抑菌谱.[结果]经不同菌源诱导黄粉虫所产生的抗菌肽抑菌活性及抑菌谱上均存在明显差异.[结论]生防菌源在诱导昆虫体液免疫物质--抗菌肽表达上具有明显优势.
[목적]탐색경불동균원유도황분충소산생적면역물질--항균태대병원균시부존재활성차이.[방법]대기아후황분충유충통과사세균、침자사진균(방선균)법산생유도면역반응,연마、냉동리심법획득항균태제취물,채용억균권법측정기대26충병원균억균활성,이용삼등분법화분사충류형평개억균활성급억균보.[결과]경불동균원유도황분충소산생적항균태억균활성급억균보상균존재명현차이.[결론]생방균원재유도곤충체액면역물질--항균태표체상구유명현우세.
[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers. [Method] By inducing T. molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking, the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures. Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring, and evaluated by trisection to four types and inhibitory spectrum. [Result] Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers. [Conclusion] Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.