CAJ | 학술논문

目的探讨荧光定量聚合酶链反应(FQ-PCR)检测宫颈病变人乳头瘤病毒(HPV)13种高危型DNA的可行性,为检测宫颈组织中HPV的感染提供有效的方法.方法选择197例阴道细胞学检测异常的宫颈脱落细胞,使用FQ-PCR方法检测13种高危型HPV DNA,同时取宫颈病变处组织进行组织病理学检查.结果 329例样本中HPV 13种高危型阳性者218例,阳性率为66.26%:CT值范围在13～40之间,平均为(26±2.31);组织病理学检测186例阳性,阳性率为56.53%,经x~2检验,差异有统计学意义(x~2=6.566,P=0.0104);阴性标本为Undet.结论 FQ-PCR方法检测宫颈病变中13种高危型HPV DNA感染,具有快速、简便、灵敏度高、特异性强等优点,对宫颈癌的普查和治疗有指导意义.
목적 탐토형광정량취합매련반응(FQ-PCR)검측궁경병변인유두류병독(HPV)13충고위형DNA적가행성,위검측궁경조직중HPV적감염제공유효적방법.방법 선택197례음도세포학검측이상적궁경탈락세포,사용FQ-PCR방법검측13충고위형HPV DNA,동시취궁경병변처조직진행조직병이학검사.결과 329례양본중HPV 13충고위형양성자218례,양성솔위66.26%:CT치범위재13～40지간,평균위(26±2.31);조직병이학검측186례양성,양성솔위56.53%,경x~2검험,차이유통계학의의(x~2=6.566,P=0.0104);음성표본위Undet.결론 FQ-PCR방법검측궁경병변중13충고위형HPV DNA감염,구유쾌속、간편、령민도고、특이성강등우점,대궁경암적보사화치료유지도의의.
Objective Quantitative detected 13 types of high risk HPV DNA in cervical lesions by FQ-PCR,in order to establish a quick and sensitive test to detect HPV in cervical lesions.Methods 13 types of high risk HPV DNA were detected in 329 cases of cervical lesions by FQ-PCR.HPV was also detected by histopathological examination.Results The positive rate of 13 types of high risk HPV DNA was 66.26%by FQ-PCR.The Ct(Threshold Cycle)value scope between 13～40,and the average value was 26±2.31.There were Undet in negative cases.The positive rate of HPV was 56.53% by histopathological examination.There was a significant difference between FQ-PCR and histopathological examination(x~2=6.566,P=0.010 4).Conclusion FQPCR is a valuable method for quantitative detection 13 types of high risk HPV DNA in cervical lesions.There are simple,the sensiivity to be high fast,specificity strong and so on merits,has the guiding sense to cervical cancer's general survey and the treatment.