CAJ | 학술논문

背景:近年来牙再生成为组织工程领域一项重要课题,但目前还没有最合适的种子细胞,迫切需要探索新的种子细胞.目的:综述脐血干细胞作为牙再生种子细胞的可行性.方法:应用计算机检索1998-01/2009-01 PubMed数据库相关文章,检索词为"tooth regeneration, seed cells, tissue engineering, umbilical cord blood,mesenchymal stem cells",并限定文章语言种类为English.同时计算机检索1998-01/2009-01中国期刊网全文数据库、万方数据库、中文科技期刊全文数据库相关文章,检索词为"组织工程,种子细胞,间充质干细胞,脐带血,牙再生",并限定文章语言种类为中文.共检索到文献40篇.结果与结论:各类种子细胞,如牙胚来源的成牙本质细胞,牙髓和乳牙来源的干细胞,以及第一腮弓来源的外胚层间充质干细胞都已经在基础研究中显示出了成牙分化潜能.但是,在临床实验中这些种子细胞很难获取.骨髓间充质干细胞虽然也显示出了成牙潜能,但是它们的分化能力随供体年龄的增长显著下降.脐血干细胞和骨髓间充质干细胞相比,拥有很多优势,并且呈现出典型的间充质干细胞的特点.所以,推测脐血干细胞可能被诱导成成牙细胞系,并且可能成为合适的牙再生的种子细胞来修复牙缺失.
배경:근년래아재생성위조직공정영역일항중요과제,단목전환몰유최합괄적충자세포,박절수요탐색신적충자세포.목적:종술제혈간세포작위아재생충자세포적가행성.방법:응용계산궤검색1998-01/2009-01 PubMed수거고상관문장,검색사위"tooth regeneration, seed cells, tissue engineering, umbilical cord blood,mesenchymal stem cells",병한정문장어언충류위English.동시계산궤검색1998-01/2009-01중국기간망전문수거고、만방수거고、중문과기기간전문수거고상관문장,검색사위"조직공정,충자세포,간충질간세포,제대혈,아재생",병한정문장어언충류위중문.공검색도문헌40편.결과여결론:각류충자세포,여아배래원적성아본질세포,아수화유아래원적간세포,이급제일시궁래원적외배층간충질간세포도이경재기출연구중현시출료성아분화잠능.단시,재림상실험중저사충자세포흔난획취.골수간충질간세포수연야현시출료성아잠능,단시타문적분화능력수공체년령적증장현저하강.제혈간세포화골수간충질간세포상비,옹유흔다우세,병차정현출전형적간충질간세포적특점.소이,추측제혈간세포가능피유도성성아세포계,병차가능성위합괄적아재생적충자세포래수복아결실.
BACKGROUND: With the development of tissue engineering, more attention has been paid to tooth regeneration. However, the resource of best seed cells is still uncertain. Therefore, alternative sources should be attached to intensive investigation. OBJECTIVE: To review the feasibility of stem cells from umbilical cord blood as seed cells for tooth regeneration. METHODS: A PubMed search was performed for articles published between January 1998 and January 2009. Key words were "tooth regeneration, seed cells, tissue engineering, umbilical cord blood, mesenchymal stem cells". Only studies written in English were included. Simultaneously, databases of China National Knowledge Infrastructure, Wanfang, and VIP published between January 1998 and January 2009 were also retrieved, using the key words of "tooth regeneration, seed cells, tissu engineering, umbilical cord blood, mesenchymal stem cells". Only studies written in Chinese were included. Totally, 40 literatures were included. RESULTS AND CONCLUSION: Seed cells, such as odontoblasts from dental germ, stem cells from dental pulp and deciduous teeth, and ectomesenchymal cells from the firstbranchial arch showed odontogenic differentiation potential in basic research. However, these cells are not practical to harvest in clinic. Although bone marrow mesenchymal stem cells have odontogenic capacity, their differentiation abilities significantly decrease with the increasing age of the donors. Stem cells from umbilical cord blood have many clinical advantages over bone marrow mesenchymal stem cells, and exhibited typical mesenchymal stem cells characteristics. Thus, we propose the hypotheses that stem cells from umbilical cord blood could be induced into odontogenic lineage and might be used as suitable seed cells for tooth regeneration to replace the lost tooth.