CAJ | 학술논문

[目的]对牛SLC27A1基因进行SNPs筛选并与中国荷斯坦奶牛的产奶性状进行关联分析,以期发现对中国荷斯坦奶牛产奶性状有显著影响的SNP位点.[方法]根据性能测定记录选取48头中国荷斯坦奶牛,提取血样DNA,组成2个DNA池,用于SNPs筛选,采用PCR-SSCP和克隆测序方法对DNA池进行SLC27A1基因SNPs筛选.针对发现的SNP位点,采用PCR-RFLP方法对另外231头中国荷斯坦奶牛进行群体基因型检测,采用SAS(8.02)GLM过程对各基因型与产奶性状进行关联分析.[结果]在exon3发现T112C位点,在3'UTR 发现G64A位点,T112C为同义突变;经PCR-RFLP检测,发现在T112C位点存在TT、TC、CC 3种基因型,G64A位点存在GG、GA、AA 3种基因型.2个位点均处于Hardy-Weinberg平衡状态.T112C位点的CC型个体的产奶量极显著高于TC型个体(P<0.01),3种基因型对乳蛋白率和乳脂率影响均不显著(P>0.05),乳蛋白率呈现CC>TC>TT的趋势,乳脂率呈现TT>TC>CC的趋势;G64A位点3种基因型对产奶量、乳蛋白率、乳脂率的影响均不显著(P>0.05),但产奶量呈现GA>GG>AA的趋势,乳蛋白率和乳脂率呈现AA>GG>GA的趋势.[结论]该基因T112C位点与产奶量性状有一定的关联性,通过提高CC基因型频率有望提高中国荷斯坦奶牛的产奶量,SLC27A1基因可作为调控中国荷斯坦奶牛产奶量的候选基因,同时为该基因的标记辅助育种和进一步研究奠定了良好基础.
[목적]대우SLC27A1기인진행SNPs사선병여중국하사탄내우적산내성상진행관련분석,이기발현대중국하사탄내우산내성상유현저영향적SNP위점.[방법]근거성능측정기록선취48두중국하사탄내우,제취혈양DNA,조성2개DNA지,용우SNPs사선,채용PCR-SSCP화극륭측서방법대DNA지진행SLC27A1기인SNPs사선.침대발현적SNP위점,채용PCR-RFLP방법대령외231두중국하사탄내우진행군체기인형검측,채용SAS(8.02)GLM과정대각기인형여산내성상진행관련분석.[결과]재exon3발현T112C위점,재3'UTR 발현G64A위점,T112C위동의돌변;경PCR-RFLP검측,발현재T112C위점존재TT、TC、CC 3충기인형,G64A위점존재GG、GA、AA 3충기인형.2개위점균처우Hardy-Weinberg평형상태.T112C위점적CC형개체적산내량겁현저고우TC형개체(P<0.01),3충기인형대유단백솔화유지솔영향균불현저(P>0.05),유단백솔정현CC>TC>TT적추세,유지솔정현TT>TC>CC적추세;G64A위점3충기인형대산내량、유단백솔、유지솔적영향균불현저(P>0.05),단산내량정현GA>GG>AA적추세,유단백솔화유지솔정현AA>GG>GA적추세.[결론]해기인T112C위점여산내량성상유일정적관련성,통과제고CC기인형빈솔유망제고중국하사탄내우적산내량,SLC27A1기인가작위조공중국하사탄내우산내량적후선기인,동시위해기인적표기보조육충화진일보연구전정료량호기출.
[Objective] This study discussed the SNPs of SLC27A1 gene and its relationship with milk production traits in Chinese Holstein Cattle in order to find the SNP site which had significant effect on milk production traits in Chinese Holstein Cattle. [Method] DNA was extracted from the bleed of 48 Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNPs detection by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and cloning sequencing. Then different genotypes were detected in other 231 Chinese Holstein Cattle by PCR-RFLP. The association between genotype and production traits was assessed by GLM procedure, SAS version 8.02. [Result] There were T112C in exon3 and G64A loci in 3'UTR, among them the T112C in exon3 was synonymous mutation. There were 3 genotypes TT, TC and CC in T112C locus and 3 genotypes GG, GA and AA in G64A locus. The population was at Hardy-Weinberg equilibration. Cows with genotype CC had significantly highest milk yield than those with genotype TC (P<0.01), and there were no significant differences among the 3 genotypes on milk protein percent and milk fat percent (P>0.05), but the tendency of CC > TC > TT on milk protein percent and the tendency of TT > TC > CC on milk fat percent were showed. There was no significant difference among the 3 genotypes of G64A loci on milk yield, milk protein percent and milk fat percent (P>0.05), but the tendency of GA > GG > AA on milk yield and the tendency of AA > GG > GA both on milk protein percent and milk fat percent were showed. [Conclusion] There was certain relation between the T112C locus and milk yield traits; It may improve milk yield to raise the frequency of genotype CC; SLC27A1 gene could be a useful candidate gene in selection program on milk yield traits in Chinese Holstein Cattle, which provided a theoretical basis for the marker-assisted breeding and further study of SLC27A1 gene.