CAJ | 학술논문

目的观察前胡甲素(Pd-Ia)对小鼠局灶性脑缺血损伤的保护作用及特点.方法线栓法制备小鼠大脑中动脉栓塞脑缺血损伤模型.Pd-Ia(1,5,10 ms/kg)在缺血前0.5 h腹腔给药1次;或在缺血前1,0.5 h、缺血同时、再灌注同时、再灌后0.5 h及再灌后1 h各腹腔给予Pd-Ia 5 ms/kg.脑缺血1.5 h,再灌注24 h后,测定小鼠神经功能缺失评分、脑梗死体积、脑水肿等评定脑缺血损伤的指标;测定血清中丙二醛(MDA)和超氧化物岐化酶(SOD)的活性.结果 Pd-Ia(5,10ms/kg)缺血前0.5 h给药及Pd-Ia 5 ms/ks缺血前0.5 h、缺血同时、再灌注同时及再灌后0.5h给药可明显改善小鼠神经功能损伤,减小脑梗死体积和减轻脑水肿程度,且以再灌注同时单次给药效果最为显著;Pd-Ia(5,10 ms/ks)能够明显提高脑缺血损伤小鼠血清中SOD活性,降低MDA含量.结论 Pd-Ia保护小鼠局灶性脑缺血急性损伤,最佳剂量为5 ms/ks,最佳治疗时间点为再灌注同时;其保护脑缺血损伤的机制可能与押制脂质过氧化、提高氧化酶的活性有关.
목적 관찰전호갑소(Pd-Ia)대소서국조성뇌결혈손상적보호작용급특점.방법 선전법제비소서대뇌중동맥전새뇌결혈손상모형.Pd-Ia(1,5,10 ms/kg)재결혈전0.5 h복강급약1차;혹재결혈전1,0.5 h、결혈동시、재관주동시、재관후0.5 h급재관후1 h각복강급여Pd-Ia 5 ms/kg.뇌결혈1.5 h,재관주24 h후,측정소서신경공능결실평분、뇌경사체적、뇌수종등평정뇌결혈손상적지표;측정혈청중병이철(MDA)화초양화물기화매(SOD)적활성.결과 Pd-Ia(5,10ms/kg)결혈전0.5 h급약급Pd-Ia 5 ms/ks결혈전0.5 h、결혈동시、재관주동시급재관후0.5h급약가명현개선소서신경공능손상,감소뇌경사체적화감경뇌수종정도,차이재관주동시단차급약효과최위현저;Pd-Ia(5,10 ms/ks)능구명현제고뇌결혈손상소서혈청중SOD활성,강저MDA함량.결론 Pd-Ia보호소서국조성뇌결혈급성손상,최가제량위5 ms/ks,최가치료시간점위재관주동시;기보호뇌결혈손상적궤제가능여압제지질과양화、제고양화매적활성유관.
purpose To investigate the protective effect and character of dl-praeruptorin A(Pd-Ia)on focal cerebral ischemia in mice.Methods Transient focal cerebral ischemia in mice WaS induced by middle cerebral artery occlusion for 1.5 h.Pd-Ia was administered intraperitoneally either with multiple doses(1,5 and 10ms/ks)at 0.5 h before ischemia or single dose(5 ms/kg)at 0.5 h and 1 h before ischemic,the same time of ischemia,the same time of reperfusion,or 0.5 h and 1 h after reperfusion respectively.Neurological deficit score,infarct volume,brain edema,the activities of SOD and the contents of MDA were determined.Results Pretreatment with multiple doses(5 and 10 ms/ks)of Pd-Ia at 0.5 h before ischemia or single dose(5 mg/kg)of Pd-Ia at 0.5 h before ischemia,at the same time of ischemic,at the same time of reperfusion and 0.5 h after reperfusion significantly attenuated neurological deficit score,decreased infarct volume and alleviated brain edema,and the treatment at the time of reperfusion had the most marked effect.Pd-Ia(5 or 10 ms/ks)can significantly enhance the activities of SOD and lower the contents MDA.Conclusion dl-praeruptorin A has a neuroprotective effect on the injury in the acute phase of transient focal cerebral ischemia in mice,with optimal doses of 5 ms/ks and the optimal therapeutic time point of the same time of reperfusion.