CAJ | 학술논문

[目的]探讨除草剂对金鱼血清EST同工酶的影响.[方法]根据丁草胺、百草枯、2,4-D丁酯的24 h半数致死剂量(LC_(50)),设0.5、1.0、1.5、 2.0 ml/L稀释浓度组,并以0.1 ml/g剂量腹腔注射金鱼,采用聚丙烯酰胺凝胶电泳法研究3种除草剂对金鱼血清酯酶同工酶表达的影响.[结果]与对照相比,3种除草剂4个浓度组对金鱼血清EST的活性均有明显影响,低浓度时EST酶活性被抑制,高浓度时EST酶活性增强或消失,染毒剂量和酶活性存在剂量-效应关系.[结论]3种除草剂对金鱼血清酯酶同工酶具有一定影响,其中百草枯对金鱼同工酶的毒性最强,2,4-D丁酯次之,丁草胺最弱.
[목적]탐토제초제대금어혈청EST동공매적영향.[방법]근거정초알、백초고、2,4-D정지적24 h반수치사제량(LC_(50)),설0.5、1.0、1.5、 2.0 ml/L희석농도조,병이0.1 ml/g제량복강주사금어,채용취병희선알응효전영법연구3충제초제대금어혈청지매동공매표체적영향.[결과]여대조상비,3충제초제4개농도조대금어혈청EST적활성균유명현영향,저농도시EST매활성피억제,고농도시EST매활성증강혹소실,염독제량화매활성존재제량-효응관계.[결론]3충제초제대금어혈청지매동공매구유일정영향,기중백초고대금어동공매적독성최강,2,4-D정지차지,정초알최약.
[Objective]The research aimed to explore the effects of herbicide on the expression of EST isozyme.[Method]Three kinds of herbicide(butachlor,paraquat,2,4-D butylate) were adopted,and 0.5 ml/L,1.0 ml/L,1.5 ml/L,2.0 ml/L concentrations each of them were prepared according 24 h LC_(50).Goldfish was injected with 0.1 ml/g dose.SDS-polyarylamidegel electrophoresis test was used to study the toxicity on expression of EST.[Result]Compared with the control,four concentration groups showed significant variety.The activity of EST reduced at low concentration,and the activity increased or disappeared at high concentration.Concentration and activity had dose-effect relationship.[Conclusion]Three kinds of herbicide had toxicity on EST isozymes.The activity of different herbicide was different.