CAJ | 학술논문

目的:观察探索学习对局灶性脑梗死大鼠巢蛋白(nestin)及神经生长因子(NGF)表达的影响.方法:健康雄性SD大鼠70只,其中60只以电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索学习组(n=30)居于探索笼,对照组(n=30)每5只一组群居于标准笼,假手术组(n=10)仅开颅不电凝大脑中动脉,居于标准笼.探索学习组和对照组分别于术后第1天、第1周、第2周、第4周各组随机选取5只大鼠处死,假手术组分别于MCAO第1周、第4周时随机选取5只大鼠处死,即进行巢蛋白及NGF免疫组化染色,测定其在脑梗死灶周围皮质巢蛋白及NGF的表达情况.结果:探索学习组巢蛋白及NGF阳性神经元数在MCAO术后第7天、第14天明显多于手术对照组(P＜0.05).结论:探索学习能促进梗死灶周围皮质巢蛋白及NGF的表达.
목적:관찰탐색학습대국조성뇌경사대서소단백(nestin)급신경생장인자(NGF)표체적영향.방법:건강웅성SD대서70지,기중60지이전응법조성우측대뇌중동맥조단(MCAO)모형후,수궤분위탐색학습조(n=30)거우탐색롱,대조조(n=30)매5지일조군거우표준롱,가수술조(n=10)부개로불전응대뇌중동맥,거우표준롱.탐색학습조화대조조분별우술후제1천、제1주、제2주、제4주각조수궤선취5지대서처사,가수술조분별우MCAO제1주、제4주시수궤선취5지대서처사,즉진행소단백급NGF면역조화염색,측정기재뇌경사조주위피질소단백급NGF적표체정황.결과:탐색학습조소단백급NGF양성신경원수재MCAO술후제7천、제14천명현다우수술대조조(P＜0.05).결론:탐색학습능촉진경사조주위피질소단백급NGF적표체.
Objective: To study the effects of learning on nestin and nerve growth factor (NGF) expressions in peri-ischemic cortex of rats after unilateral local cerebral infarction. Method: Seventy male Sprague-Dawley rats were adopted. After the models of middle cerebral artery occlusion (MCAO) were established by electric coagulation successfully, the rats were randomly divided into learning group(n=30, living in exploratory cages) and control group (n=30, every 5 rats as a group living in large standard cages). Other 10 rats without coagulation as sham group lived in standard cage. At the 1st, 7th, 14th, 28th d after MCAO, every 5 rats in learning group and control group were randomly sacrificed separately. At the 7th, 28th d after operation 5 rats in sham group were randomly sacrificed separately. The expressions of nestin and NGF in peri -ischemic cortex were examined by immunohistochemistry staining. Result: The nestin and NGF labeled neuron cells in learning group were more than those in control group at the 7th and 14th d after MCAO(P＜0.05). Conclusion: Learning could enhance nestin and NGF expressions in peri-ischemic cortex of rats after unilateral local cerebral infarction.