CAJ | 학술논문

采用氯仿-甲醇有机溶剂提取、常压硅胶柱层析等方法进行分离纯化,从微劳马尾藻的提取物中分离纯化了6个化合物;根据核磁共振谱分析及文献解析化合物B、D分别确定为棕榈酰基.油酰基-3.0-α-D-吡喃葡萄糖基甘油和肉豆蔻酰基.油酰基-3-O-α-D-吡喃葡萄糖基甘油;使用酶标仪体外测定化合物的纤溶活性,发现化合物B使纤溶作用提高了10%～30%,化合物D的纤溶促进作用弱于化合物B;分离纯化的纤溶促进化合物B和D属于甘油糖脂类化合物.本文首次从微劳马尾藻分离得到了肉豆蔻酰基一油酰基-3-O-α-D-吡喃葡萄糖基甘油.
채용록방-갑순유궤용제제취、상압규효주층석등방법진행분리순화,종미로마미조적제취물중분리순화료6개화합물;근거핵자공진보분석급문헌해석화합물B、D분별학정위종려선기.유선기-3.0-α-D-필남포도당기감유화육두구선기.유선기-3-O-α-D-필남포도당기감유;사용매표의체외측정화합물적섬용활성,발현화합물B사섬용작용제고료10%～30%,화합물D적섬용촉진작용약우화합물B;분리순화적섬용촉진화합물B화D속우감유당지류화합물.본문수차종미로마미조분리득도료육두구선기일유선기-3-O-α-D-필남포도당기감유.
The isolation,determination of fibrinolytic activities and structures of compounds were assayed by solvent extraction and successive chromatographic fraction using silica gel, the method of chromogenic substrate in vitro and 1D NMR(~1H,~(13)C,DEPT) and 2D NMR(~1H-~1H COSY.HSQC.HMBC), respectively. We obtained six compounds from Sar-gassum fulvellum and discovered the enhancing fibrinolytic effect of compound B elevated 10%-30% ,but compound D with a slight lower activation of fibrinolysis. By analysis of NMR spectrum compounds B and D were confirmed as palmi-toyl-oleoyl-3-O-α-D-glucopyranosylglycerol and myristoyl-oleoyl-3-O-α-D-glucopyranosylglycerol. Compounds B and D gained from Sargassum fulvellum were designated glycolipid compounds, and myristoyl-oleoyl-3-O-α-D-glucopyranosylg-lycerol was obtained from Sargassum fidvellum for the first time.