浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2009年
7期
679-681
,共3页
氧化低密度脂蛋白%血管平滑肌细胞%大鼠%TLR4%Emilinl%转化生长因子β%动脉粥样硬化
氧化低密度脂蛋白%血管平滑肌細胞%大鼠%TLR4%Emilinl%轉化生長因子β%動脈粥樣硬化
양화저밀도지단백%혈관평활기세포%대서%TLR4%Emilinl%전화생장인자β%동맥죽양경화
Oxidized - Low Density Lipoprotein Vascular Smooth Muscle Cells Rat Toll - Like Receptor 4 Emilinl Transforming Growth Factor β Atherosclerosis
目的 观察氧化低密度脂蛋白(ox-LDL)对大鼠血管平滑肌细胞(VSMCs)TLR4和Emilinl表达的影响及转化生长因子-β(TGF-β)在其中所起的作用.方法 将传至2～4代的大鼠VSMCs,用不同浓度的OX-LDL(20、50、80、100μg/ml)干预,采用RT-PCR检测TLR4和Emilinl mRNA表达变化.另设100μg/ml ox-LDL+TGF-B(0.01、0.1、1、10μg/1)培养24h,采用RT-PCR检测TLR4 mRNA表达.结果 (1)用四种不同浓度梯度的ox-LDL作用后的VSMCs,TLR4和Emilinl在mRNA水平均比空白对照组表达增加,并且呈浓度依赖性.(2)不同浓度TGF-B(0.01、0.1、1、10μg/1)作用后的ox-LDL组(100μg/ml)TLR4 mRNA表达明显下降,且呈浓度依赖性.结论 ox-LDL可诱导大鼠VSMCs TLR4和Emilinl的表达,促进炎症的发展,ox-LDL可通过使VSMCs Emilinl表达增高途径抑制TGF-β的表达,最终达到促进TLR4高表达的目的 .ox-LDL对TGF-β的抑制作用是上调VSMCs TLR4表达的重要途径之一.
目的 觀察氧化低密度脂蛋白(ox-LDL)對大鼠血管平滑肌細胞(VSMCs)TLR4和Emilinl錶達的影響及轉化生長因子-β(TGF-β)在其中所起的作用.方法 將傳至2～4代的大鼠VSMCs,用不同濃度的OX-LDL(20、50、80、100μg/ml)榦預,採用RT-PCR檢測TLR4和Emilinl mRNA錶達變化.另設100μg/ml ox-LDL+TGF-B(0.01、0.1、1、10μg/1)培養24h,採用RT-PCR檢測TLR4 mRNA錶達.結果 (1)用四種不同濃度梯度的ox-LDL作用後的VSMCs,TLR4和Emilinl在mRNA水平均比空白對照組錶達增加,併且呈濃度依賴性.(2)不同濃度TGF-B(0.01、0.1、1、10μg/1)作用後的ox-LDL組(100μg/ml)TLR4 mRNA錶達明顯下降,且呈濃度依賴性.結論 ox-LDL可誘導大鼠VSMCs TLR4和Emilinl的錶達,促進炎癥的髮展,ox-LDL可通過使VSMCs Emilinl錶達增高途徑抑製TGF-β的錶達,最終達到促進TLR4高錶達的目的 .ox-LDL對TGF-β的抑製作用是上調VSMCs TLR4錶達的重要途徑之一.
목적 관찰양화저밀도지단백(ox-LDL)대대서혈관평활기세포(VSMCs)TLR4화Emilinl표체적영향급전화생장인자-β(TGF-β)재기중소기적작용.방법 장전지2～4대적대서VSMCs,용불동농도적OX-LDL(20、50、80、100μg/ml)간예,채용RT-PCR검측TLR4화Emilinl mRNA표체변화.령설100μg/ml ox-LDL+TGF-B(0.01、0.1、1、10μg/1)배양24h,채용RT-PCR검측TLR4 mRNA표체.결과 (1)용사충불동농도제도적ox-LDL작용후적VSMCs,TLR4화Emilinl재mRNA수평균비공백대조조표체증가,병차정농도의뢰성.(2)불동농도TGF-B(0.01、0.1、1、10μg/1)작용후적ox-LDL조(100μg/ml)TLR4 mRNA표체명현하강,차정농도의뢰성.결론 ox-LDL가유도대서VSMCs TLR4화Emilinl적표체,촉진염증적발전,ox-LDL가통과사VSMCs Emilinl표체증고도경억제TGF-β적표체,최종체도촉진TLR4고표체적목적 .ox-LDL대TGF-β적억제작용시상조VSMCs TLR4표체적중요도경지일.
Objective To examine the effects of oxidized -low density lipoprotein (ox - LDL) and transforming growth factor - on expressions of Toll -like receptor 4(TLR4) and Emi[inl in cultured rat vascular smith muscle cells (VSMCs). Methods Rat VSMCs were incubated with different concentrations of ox - LDL(20,50,80,100μg/ml) ,TLR4 and Emilinl mRNA expressions were detected by RT-PCR. The second group was incubated with different concentrtions of TGF - βSand ox - LDL(100μg/ml). Results The mRNA expressions of TLR4 and Emilinl were concentration - dependently increased "after VSMCs were stimulated by diferent concentrations of ox - LDL for 24h ,and the mRNA expressions of TLR4 were concentration - dependently decreased after VSMCs were stimulated by diferent concentrations of TGF -βand ox - LDL(100μg/ml) for 24h. Conclusions Ox - LDL could increase the expression activities of TLR4 and Emilinl promotes the inflanmmtory progress. It decreases the TGFβexpression,then up -regulates the expression of TLR4 by increasing the expression of Emilinl, which is a main path for un - regulating VSMCs TLR4's expression.
