CAJ | 학술논문

[目的]分析4个飞蝗亚种间的系统发育关系.[方法]利用PCR扩增技术测定了西藏飞蝗(L.migratoria tibetensis)和东亚飞蝗(L.m.manilensis)细胞色素氧化酶3亚基基因序列(COⅠ1 539 bp、COⅡ684 bp、COⅢ792bp,共计3 015 bp),结合GenBank下载的亚洲飞蝗(L.m.migratoria)和非洲飞蝗(L.m.migratorioides)的细胞色素氧化酶3亚基基因序列进行综合分析.以云斑车蝗(G.marmoratus)为外群,通过邻接法、最大简约法和贝叶斯法重建4个飞蝗亚种的分子系统树.[结果]在碱基组成上,A+T的平均含量为69.57%,4个飞蝗亚种的密码子第3位点A+T含量最高,其中最高的是COⅠ(达87.6%);4个飞蝗亚种中核苷酸替换主要发生在密码子第3位,COⅡ的核酸替代率最高,第2位点较为保守,替换率在5.9%～15%之间;C0I的起始密码子是特殊的CCG或ACG;4个飞蝗亚种的遗传距离在0.001～0.076之间;飞蝗属中4个亚种之间的系统发育关系为东亚飞蝗和西藏飞蝗亲缘关系最近,亚洲飞蝗与东亚飞蝗、西藏飞蝗亲缘关系次之,非洲飞蝗与其余3个亚种的亲缘关系较远.[结论]该研究可为西藏飞蝗亚种地位的确定提供分子依据.
[목적]분석4개비황아충간적계통발육관계.[방법]이용PCR확증기술측정료서장비황(L.migratoria tibetensis)화동아비황(L.m.manilensis)세포색소양화매3아기기인서렬(COⅠ1 539 bp、COⅡ684 bp、COⅢ792bp,공계3 015 bp),결합GenBank하재적아주비황(L.m.migratoria)화비주비황(L.m.migratorioides)적세포색소양화매3아기기인서렬진행종합분석.이운반차황(G.marmoratus)위외군,통과린접법、최대간약법화패협사법중건4개비황아충적분자계통수.[결과]재감기조성상,A+T적평균함량위69.57%,4개비황아충적밀마자제3위점A+T함량최고,기중최고적시COⅠ(체87.6%);4개비황아충중핵감산체환주요발생재밀마자제3위,COⅡ적핵산체대솔최고,제2위점교위보수,체환솔재5.9%～15%지간;C0I적기시밀마자시특수적CCG혹ACG;4개비황아충적유전거리재0.001～0.076지간;비황속중4개아충지간적계통발육관계위동아비황화서장비황친연관계최근,아주비황여동아비황、서장비황친연관계차지,비주비황여기여3개아충적친연관계교원.[결론]해연구가위서장비황아충지위적학정제공분자의거.
[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta. [Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced ( COⅠ 1 539 bp, COⅡ 684 bp, CO Ⅲ 792 bp, with the total of 3 015 bp). The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment. Phylogenic trees of four subspecies of L. migratoria were constructed by Neighbor-Joining, Maximum-parsimony and Bayesian, respectively. [Result] The average content of A + T in three subunits of four subspecies was 69.57%; the third site of codon showed the highest A + T content, and the COⅠ had the highest A + T content (87.6%); The nucleotide substitution mainly occurred at the third site of codon, and the nucleotide replacement rate of CO Ⅱ was the highest. The second site of codon was conservative, so the replacement rate was in the range of 5.9% - 15%. The start codon of COⅠ was CCG or ACG. Genetic distances among four subspecies were ranged from 0.001 to 0. 076. The relationship between L. m. tibetensis and Locusta migratoria manilensis was the closest, followed by L. m. migratorioides and L. m. migratorioides, while the genetic distance between L. m. tibetensis and L. m. migratorioides was the largest. [Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L. m. tibetensis, L. m. manilensis, L. m. migratoria, L. m. migratorioides.