CAJ | 학술논문

目的初步探讨人趋化因子样因子-1 (human chemokine-like factor-1，hCKLF-1)在系统性红斑狼疮BXSB小鼠发病中的作用。方法利用肌肉介导的电脉冲转基因技术，便重组的真核表达质粒pCDI-hCKLF-1在BXSB小鼠体内表达，并检测血清中IgM和IgG型抗DNA抗体的水平，尿蛋白浓度和血清尿素氮(BUN)浓度。结果 hCKLF-1的体内表达对雌雄BXSB小鼠IgM和IgG型抗DNA抗体的产生无明显影响，但在短时间内显著地促进雄性BXSB小鼠尿蛋白浓度的异常升高，加重其肾脏炎症。结论 hCKLF-1可能是参与狼疮肾炎的介质之一，其效应细胞可能是单核巨噬细胞。
목적초보탐토인추화인자양인자-1 (human chemokine-like factor-1，hCKLF-1)재계통성홍반랑창BXSB소서발병중적작용。방법이용기육개도적전맥충전기인기술，편중조적진핵표체질립pCDI-hCKLF-1재BXSB소서체내표체，병검측혈청중IgM화IgG형항DNA항체적수평，뇨단백농도화혈청뇨소담(BUN)농도。결과 hCKLF-1적체내표체대자웅BXSB소서IgM화IgG형항DNA항체적산생무명현영향，단재단시간내현저지촉진웅성BXSB소서뇨단백농도적이상승고，가중기신장염증。결론 hCKLF-1가능시삼여랑창신염적개질지일，기효응세포가능시단핵거서세포。
Objective　To probe into roles of hCKLF-1 in the development of systemic lupus erythematosus in BXSB mice in vivo.Methods　The recombinant eukaryotic expression plasmid for hCKLF-1 (pCDI-hCKLF-1) was transferred and expressed in lupus-prone BXSB mice with the technique of muscle-mediated transgene by electric pulse;the levels of serum IgM and IgG anti-DNA antibodies as well as serum BUN and urine protein were detected.Results　hCKLF-1 expression in vivo did not make any effects on the levels of IgM and IgG anti-DNA antibodies in female and male BXSB mice,but markedly enabled abnormal elevation of urine protein in male BXSB mice in short time,suggesting its ability for accelerating glomerulonephritis.Conclusion　hCKLF-1 may be one of inflammation mediators,playing an important role in the lupus glomerulonephritis of male BXSB mice,and its target cells may be monocyte and macrophage.