CAJ | 학술논문

表皮生长因子(EGF)具有促进多种细胞增殖的作用,尤其在维持消化道黏膜完整和促进消化道溃疡愈合方面作用巨大.以前的研究多集中在细菌和酵母中表达,本研究试图以番茄作为生物反应器生产重组人EGF,使之成本降低,应用方便.依据人EGF的基因序列,设计合成了番茄密码子偏爱的人EGF基因,并将其构建到植物表达载体pCAMBIA2300中,通过农杆菌介导得到了含有人EGF基因的转基因番茄.放射免疫法检测到每克鲜重果实中的表达量达3.48±1.01 ng.将果汁灌喂小白鼠15天(相当于每鼠每天喂服24 ng rhEGF)能显著抵抗酒精引起的胃溃疡形成,溃疡指数由42.20±18.13下降为16.25±9.57.
표피생장인자(EGF)구유촉진다충세포증식적작용,우기재유지소화도점막완정화촉진소화도궤양유합방면작용거대.이전적연구다집중재세균화효모중표체,본연구시도이번가작위생물반응기생산중조인EGF,사지성본강저,응용방편.의거인EGF적기인서렬,설계합성료번가밀마자편애적인EGF기인,병장기구건도식물표체재체pCAMBIA2300중,통과농간균개도득도료함유인EGF기인적전기인번가.방사면역법검측도매극선중과실중적표체량체3.48±1.01 ng.장과즙관위소백서15천(상당우매서매천위복24 ng rhEGF)능현저저항주정인기적위궤양형성,궤양지수유42.20±18.13하강위16.25±9.57.
The epidermal growth factor (EGF) has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the gastric and duodenal ulcers. It has been expressed in many types of bacteria and yeasts. In this article, a bio-reactor was constructed, namely, the human EGF (hEGF)transgenic mini-tomato. On the basis of hEGF gene sequence, a tomato codon preference hEGF gene was chemically synthesized,and it was constructed into the plant expression vector pCAMBIA2300. The transgenic tomato plants containing gene hEGF were obtained through Agrobacterium-mediated transformation. The expression product was determined by radioimmunoassay (RIA) and showed a yield of 3.48 ± 1.01 ng/g fresh fruits. The intragastric gavage (ig) administration of the rhEGF-containing juice of the transgenic tomato (equivalent to 24 ng rhEGF per mouse a day) for 15 days could significantly protect mice against the alcohol-induced ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 42.20 ± 18.13 to 16.25 ± 9.57.