CAJ | 학술논문

USSR5为极早熟的前苏联品种,以抽穗期近等基因系和抽穗期QTL近等基因系为测验品种,对USSR5的抽穗期基因型进行分析,表明USSR5携带了非感光基因e1、无感光功能的Se-1e基因、感光抑制基因i-Se-1和显性早熟基因Ef-1,从而使它表现极早熟的特性.此外,本研究调查了USSR5和N22的BC1F1和F2群体的抽穗期,利用Windows QTL Cartographer1.13a软件,采用复合区间作图法,在全基因组范围内,分析了南京夏季正常日照条件下2个群体的抽穗期QTL,在USSR5/N22//USSR5 BC1F1群体,共检测到2个位点,分别位于第7、8染色体上,其LOD值分别是6.11和2.91,对表型总变异的解释率分别为27.38%和11.15%,2个位点上来自USSR5的等位基因均提早抽穗.在USSR5/N22 F2群体,共检测到5个位点,分别位于第1、2、7、9、10染色体上.5个位点LOD值介于3.02～8.4,对表型总变异的解释率分别为4.07%和15.41%.除qHd-9外,其余控制抽穗期的4个基因位点上提早抽穗的等位基因均来源于USSR5.比较分析发现效应较大的qHd-7即是Hd4(E1),USSR5在该位点上携带非感光基因hd4(e1).尽管本研究定位的其它抽穗期QTL和已知抽穗期基因之间尚不能一一对应,但在早熟性水稻品种选育中,USSR5将可作为良好的基因源加以利用.
USSR5위겁조숙적전소련품충,이추수기근등기인계화추수기QTL근등기인계위측험품충,대USSR5적추수기기인형진행분석,표명USSR5휴대료비감광기인e1、무감광공능적Se-1e기인、감광억제기인i-Se-1화현성조숙기인Ef-1,종이사타표현겁조숙적특성.차외,본연구조사료USSR5화N22적BC1F1화F2군체적추수기,이용Windows QTL Cartographer1.13a연건,채용복합구간작도법,재전기인조범위내,분석료남경하계정상일조조건하2개군체적추수기QTL,재USSR5/N22//USSR5 BC1F1군체,공검측도2개위점,분별위우제7、8염색체상,기LOD치분별시6.11화2.91,대표형총변이적해석솔분별위27.38%화11.15%,2개위점상래자USSR5적등위기인균제조추수.재USSR5/N22 F2군체,공검측도5개위점,분별위우제1、2、7、9、10염색체상.5개위점LOD치개우3.02～8.4,대표형총변이적해석솔분별위4.07%화15.41%.제qHd-9외,기여공제추수기적4개기인위점상제조추수적등위기인균래원우USSR5.비교분석발현효응교대적qHd-7즉시Hd4(E1),USSR5재해위점상휴대비감광기인hd4(e1).진관본연구정위적기타추수기QTL화이지추수기기인지간상불능일일대응,단재조숙성수도품충선육중,USSR5장가작위량호적기인원가이이용.
USSR5, a japonica rice variety from the former Soviet Union, is an extremely early maturing rice variety.To elucidate the genetic basis for its early heading, genetic analysis was carried out by crossing it with a set of major gene nearly isogenic lines(NIL) and QTL-isogenic lines.The early heading of USSR5 was attributed to the presence of photoperiod-insensitive alleles at E1 and Se-1 gene, the photoperiod-sensitive inhibitor gene i-Se-1, and the dominant earliness gene Ef-1.Analysis of a backcrossed population (BC1F1) derived from the cross USSR5×N22 indicated that two quantitative trait loci (QTL) for early heading were located on chromosomes 7 and 8, accounting for 27.4% and 11.2% of the phenotypic variance, respectively, with both early alleles originating from USSR5.From an F2 population of the same cross, early heading QTLs were detected on chromosomes 1, 2, 7, 9,and 10, with individual QTL accounting for between 4.1% and 15.4% of the phenotypic variance.Early heading alleles at four of these five QTLs originated fr om USSR5.A comparison of chromosomal locations suggests that one of these QTLs may be identical with the known gene Hd4 (E1).The relationship between the other QTLs and known genes for heading date are not clear.USSR5 is a promising source for propagating earliness for the development of improved early heading rice varieties.