CAJ | 학술논문

背景:中药红景天、人参、当归的有效成分具有抗缺氧损伤的作用.目的:从细胞电生理角度观察中药芎归滴丸对脑海马神经元的保护作用.设计:以细胞为观察对象,自身对照观察.单位:解放军第八十八医院和解放军军事医学科学院.材料:实验于2002-03/08在解放军军事医学科学院毒物药物研究所完成.选择新生清洁级Wistar大鼠1只,取其海马,将其分离培养成海马神经元细胞,然后选取9例海马神经元细胞,将其放入3个培养皿中培养,每个培养皿中3例神经元细胞,将其分为缺氧-复氧前后(对照组)、芎归滴丸(由解放军第八十八医院制剂室提供,主要成分为川芎和当归的挥发油)1×10-6mol/L组和芎归滴丸10×10-6 mol/L组.方法:缺氧-复氧前后组分为两个亚组(即缺氧-复氧前、缺氧-复氧后),均不进行药物干预;芎归滴丸1×10-6 mol/L组分为两个亚组(即芎归滴丸1.0×10-6mol/L+不缺氧组、芎归滴丸1×10-6mol/L+缺氧组);芎归滴丸10×10-6mol/L组分为两个亚组(即芎归滴丸10×10-6 mol/L+不缺氧组、芎归滴丸10×10-6mol/L+缺氧组).采用膜片箝全细胞记录方法检测缺氧-复氧前后体外培养的大鼠海马神经元的电压门控性Na+、K+电流.主要观察指标:体外培养的大鼠海马神经元的电压门控性Na+、K+电流幅度.结果:①海马神经元的电压门控性Na+电流幅度变化:缺氧-复氧后明显低于缺氧-复氧前[(-3.8±1.0,-10.3±0.5)nA,t=3.49,P＜0.01].②海马神经元的电压门控性K+电流幅度变化:缺氧-复氧后K+电流的激活阈值由-40 mV变为-20 mV,电流幅度变化差异无显著性意义.③芎归滴丸预处理后缺氧-复氧前后海马神经元的Na+、K+变化:缺氧-复氧前海马神经元Ⅰ Na Ⅰk在阈值处的幅度很接近,差异无显著性意义.缺氧-复氧后芎归滴丸10×10-6mol/L组高于芎归滴丸1×10-6mol/L组和缺氧-复氧后[(-58.5±1.9,-6.9±1.4,-3.8±1.0)nA,t=7.51,P＜0.05].结论:芎归滴丸对海马神经元的缺氧损伤有抑制作用. 揹景:中藥紅景天、人參、噹歸的有效成分具有抗缺氧損傷的作用.目的:從細胞電生理角度觀察中藥芎歸滴汍對腦海馬神經元的保護作用.設計:以細胞為觀察對象,自身對照觀察.單位:解放軍第八十八醫院和解放軍軍事醫學科學院.材料:實驗于2002-03/08在解放軍軍事醫學科學院毒物藥物研究所完成.選擇新生清潔級Wistar大鼠1隻,取其海馬,將其分離培養成海馬神經元細胞,然後選取9例海馬神經元細胞,將其放入3箇培養皿中培養,每箇培養皿中3例神經元細胞,將其分為缺氧-複氧前後(對照組)、芎歸滴汍(由解放軍第八十八醫院製劑室提供,主要成分為川芎和噹歸的揮髮油)1×10-6mol/L組和芎歸滴汍10×10-6 mol/L組.方法:缺氧-複氧前後組分為兩箇亞組(即缺氧-複氧前、缺氧-複氧後),均不進行藥物榦預;芎歸滴汍1×10-6 mol/L組分為兩箇亞組(即芎歸滴汍1.0×10-6mol/L+不缺氧組、芎歸滴汍1×10-6mol/L+缺氧組);芎歸滴汍10×10-6mol/L組分為兩箇亞組(即芎歸滴汍10×10-6 mol/L+不缺氧組、芎歸滴汍10×10-6mol/L+缺氧組).採用膜片箝全細胞記錄方法檢測缺氧-複氧前後體外培養的大鼠海馬神經元的電壓門控性Na+、K+電流.主要觀察指標:體外培養的大鼠海馬神經元的電壓門控性Na+、K+電流幅度.結果:①海馬神經元的電壓門控性Na+電流幅度變化:缺氧-複氧後明顯低于缺氧-複氧前[(-3.8±1.0,-10.3±0.5)nA,t=3.49,P＜0.01].②海馬神經元的電壓門控性K+電流幅度變化:缺氧-複氧後K+電流的激活閾值由-40 mV變為-20 mV,電流幅度變化差異無顯著性意義.③芎歸滴汍預處理後缺氧-複氧前後海馬神經元的Na+、K+變化:缺氧-複氧前海馬神經元Ⅰ Na Ⅰk在閾值處的幅度很接近,差異無顯著性意義.缺氧-複氧後芎歸滴汍10×10-6mol/L組高于芎歸滴汍1×10-6mol/L組和缺氧-複氧後[(-58.5±1.9,-6.9±1.4,-3.8±1.0)nA,t=7.51,P＜0.05].結論:芎歸滴汍對海馬神經元的缺氧損傷有抑製作用.
배경:중약홍경천、인삼、당귀적유효성분구유항결양손상적작용.목적:종세포전생리각도관찰중약궁귀적환대뇌해마신경원적보호작용.설계:이세포위관찰대상,자신대조관찰.단위:해방군제팔십팔의원화해방군군사의학과학원.재료:실험우2002-03/08재해방군군사의학과학원독물약물연구소완성.선택신생청길급Wistar대서1지,취기해마,장기분리배양성해마신경원세포,연후선취9례해마신경원세포,장기방입3개배양명중배양,매개배양명중3례신경원세포,장기분위결양-복양전후(대조조)、궁귀적환(유해방군제팔십팔의원제제실제공,주요성분위천궁화당귀적휘발유)1×10-6mol/L조화궁귀적환10×10-6 mol/L조.방법:결양-복양전후조분위량개아조(즉결양-복양전、결양-복양후),균불진행약물간예;궁귀적환1×10-6 mol/L조분위량개아조(즉궁귀적환1.0×10-6mol/L+불결양조、궁귀적환1×10-6mol/L+결양조);궁귀적환10×10-6mol/L조분위량개아조(즉궁귀적환10×10-6 mol/L+불결양조、궁귀적환10×10-6mol/L+결양조).채용막편겸전세포기록방법검측결양-복양전후체외배양적대서해마신경원적전압문공성Na+、K+전류.주요관찰지표:체외배양적대서해마신경원적전압문공성Na+、K+전류폭도.결과:①해마신경원적전압문공성Na+전류폭도변화:결양-복양후명현저우결양-복양전[(-3.8±1.0,-10.3±0.5)nA,t=3.49,P＜0.01].②해마신경원적전압문공성K+전류폭도변화:결양-복양후K+전류적격활역치유-40 mV변위-20 mV,전류폭도변화차이무현저성의의.③궁귀적환예처리후결양-복양전후해마신경원적Na+、K+변화:결양-복양전해마신경원Ⅰ Na Ⅰk재역치처적폭도흔접근,차이무현저성의의.결양-복양후궁귀적환10×10-6mol/L조고우궁귀적환1×10-6mol/L조화결양-복양후[(-58.5±1.9,-6.9±1.4,-3.8±1.0)nA,t=7.51,P＜0.05].결론:궁귀적환대해마신경원적결양손상유억제작용.
BACKGROUND: The active ingredients of Chinese herbs, such as hongjingtian (rhodiola root, Radix Rhodiolae), srenshen (ginseng, Radix Ginseng), and danggui (Chinese angelica root, Radix Angelicae Sinensis),were found to be effective to inhibit the hypoxia injury.OBJECTIVE: To observe the protecting effect of xionggui diwan (Dripping Pills) on the hippocampal neurons from hypoxia by whole-cell recording patch-clamp technique.DESIGN: An auto-control observation on cells.SETTING: The 88 Hospital of Chinese PLA and Academy of Military Medical Science of Chinese PLA.MATERIALS: The experiment was performed during March to August 2002 in the Institute of Toxicology and Pharmacology of Academy of Military Medical Sciences of Chinese PLA. The neonatal Wistar rats, clean degree, were used. The hippocampal neuron cells out of 9 rats were cultured and divided into the control groups of before and after hypoxia-oxygenation,and xionggui diwan 1 × 10-6 mol/L and 10 × 10-6 mol/L groups. (Xionggui diwan was provided by the 88 Hospital of Chinese PLA and its active ingredient was the volatile ingredient of chuanxiong and danggui.)METHODS: While hypoxia-reoxygenation groups were not given any medicine intervention as control, the treatment of xionggui diwan 1×10-6mol/L was subdivided into xionggui diwan 1 × 10-6 mol/L + non-hypoxia and xionggui diwan 1 × 10-6 mol/L + hypoxia groups, and the treatment of xionggui diwan 10 × 10-6 mol/L was subdivided into xionggui diwan 10 × 10-6 mol/L + non-hypoxia and xionggui diwan 10 × 10-6 mol/L + hypoxia groups. The patch-clamp technique was performed to determine the Na+ and K+ currents of rat hippocampal neurons cultured in vitro before and after hypoxia-reoxygenation.MAIN OUTCOME MEASURES: The Na+ and K+ current amplitude of rat hippocampal neurons cultured in vitro.RESULTS:① The Na+ current amplitude of hippocampal neurons: After hypoxia-reoxygenation, it was significantly lower than that before [(-3.8±1.0, -10.3±0.5) nA, t=3.49, P ＜ 0.01]. ② The K+ current amplitude of hippocampal neurons: After hypoxia-reoxygenation, the activation thresholdof K+ current was changed from -40 mV to -20 mV.③The Na+ and K+ currents of hippocampal neurons before and after hypoxia-reoxygenation with xionggui diwan pretreatment: Before hypoxia-reoxygenation, the ampli tudes of Ⅰ Na and J K of hippocampal neurons at the threshold point were very similar, without significant difference. After hypoxia-reoxygenation,those amplitudes of xionggui diwan 10.0 × 10-6 mol/L group were higher than that of xiongguidiwan 1.0 × 10-6 mol/L group [(-58.5±1.9, -6.9±1.4,-3.8±1.0) nA, t=7.51,P ＜ 0.05].CONCLUSION: It was suggested that xionggui diwan propect the function of hippocampal neurons from hypoxia.