中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
37期
138-140
,共3页
周爽%黄建华%方邦江%袁凌松
週爽%黃建華%方邦江%袁凌鬆
주상%황건화%방방강%원릉송
缺血再灌注%电针%一氧化氮合酶
缺血再灌註%電針%一氧化氮閤酶
결혈재관주%전침%일양화담합매
背景:诱导型一氧化氮合酶mRNA在脑缺血再灌注脑损伤中具有减轻血脑屏障的破坏,保护血管内皮和脑组织的作用.目的:观察电针水沟、内关、足三里对脑缺血再灌注大鼠海马诱导型一氧化氮合酶mRNA表达的影响.设计:随机对照实验.单位:上海中医药大学、上海市针灸经络研究所及复旦大学华山医院.方法:实验于2003-12/2004-12在上海中医药大学实验动物中心、上海市针灸经络研究所针灸免疫学实验室和复旦大学华山医院完成.40只SD大鼠随机分为4组:正常组、假手术组、模型组和电针治疗组,每组10只.用双肾双夹法复制易卒中型肾血管性高血压模型,在此基础上,运用栓线法制备大鼠局灶性脑缺血再灌注模型;假手术组除不插入栓线外,其余步骤同模型组;电针治疗组取水沟(唇裂鼻尖下1 mm正中处,向上斜刺1 mm)、双侧内关(前肢内侧,离腕关节约3 mm处的尺桡骨缝间,直刺1 mm)、双侧足三里(膝关节下侧,在腓骨小头下约5 mm处,直刺7 mm)水沟穴与右耳根部皮肤、内关与足三里接G6805电针治疗仪,连续波,频率120次/min,强度1 mA,留针30 min.缺血后即时治疗1次,再灌注后每12 h治疗1次.所有动物于再灌注后24h断头处死,取出脑组织,分离海马,应用荧光定量逆转录聚合酶链反应技术观察电针对实验性脑缺血再灌注大鼠海马诱导型一氧化氮合酶mRNA表达的影响.主要观察指标:大鼠海马诱导型一氧化氮合酶mRNA的表达.结果:40只SD大鼠均进入结果分析.大鼠海马诱导型一氧化氮合酶mRNA的表达:[1]模型组明显高于电针治疗组{[(4.85±1.29)×1 000,(3.19±1.38)×1 000]拷贝,(t=2.77,P＜0.05)};[2]模型组明显高于假手术组{[(4.85±1.29)×1 000,(4.93±2.17)×10]拷贝,(t=97.38,P＜0.01)};[3]模型组显著高于正常组{[(4.85±1.29)×1 000,(3.13±1.68)×10]拷贝,(t=11.81,P＜0.01)}.结论:电针可以显著抑制脑缺血再灌注后大鼠海马诱导型一氧化氮合酶mRNA表达,从而减少一氧化氮的产生,有助于减轻脑缺血再灌注损伤.
揹景:誘導型一氧化氮閤酶mRNA在腦缺血再灌註腦損傷中具有減輕血腦屏障的破壞,保護血管內皮和腦組織的作用.目的:觀察電針水溝、內關、足三裏對腦缺血再灌註大鼠海馬誘導型一氧化氮閤酶mRNA錶達的影響.設計:隨機對照實驗.單位:上海中醫藥大學、上海市針灸經絡研究所及複旦大學華山醫院.方法:實驗于2003-12/2004-12在上海中醫藥大學實驗動物中心、上海市針灸經絡研究所針灸免疫學實驗室和複旦大學華山醫院完成.40隻SD大鼠隨機分為4組:正常組、假手術組、模型組和電針治療組,每組10隻.用雙腎雙夾法複製易卒中型腎血管性高血壓模型,在此基礎上,運用栓線法製備大鼠跼竈性腦缺血再灌註模型;假手術組除不插入栓線外,其餘步驟同模型組;電針治療組取水溝(脣裂鼻尖下1 mm正中處,嚮上斜刺1 mm)、雙側內關(前肢內側,離腕關節約3 mm處的呎橈骨縫間,直刺1 mm)、雙側足三裏(膝關節下側,在腓骨小頭下約5 mm處,直刺7 mm)水溝穴與右耳根部皮膚、內關與足三裏接G6805電針治療儀,連續波,頻率120次/min,彊度1 mA,留針30 min.缺血後即時治療1次,再灌註後每12 h治療1次.所有動物于再灌註後24h斷頭處死,取齣腦組織,分離海馬,應用熒光定量逆轉錄聚閤酶鏈反應技術觀察電針對實驗性腦缺血再灌註大鼠海馬誘導型一氧化氮閤酶mRNA錶達的影響.主要觀察指標:大鼠海馬誘導型一氧化氮閤酶mRNA的錶達.結果:40隻SD大鼠均進入結果分析.大鼠海馬誘導型一氧化氮閤酶mRNA的錶達:[1]模型組明顯高于電針治療組{[(4.85±1.29)×1 000,(3.19±1.38)×1 000]拷貝,(t=2.77,P＜0.05)};[2]模型組明顯高于假手術組{[(4.85±1.29)×1 000,(4.93±2.17)×10]拷貝,(t=97.38,P＜0.01)};[3]模型組顯著高于正常組{[(4.85±1.29)×1 000,(3.13±1.68)×10]拷貝,(t=11.81,P＜0.01)}.結論:電針可以顯著抑製腦缺血再灌註後大鼠海馬誘導型一氧化氮閤酶mRNA錶達,從而減少一氧化氮的產生,有助于減輕腦缺血再灌註損傷.
배경:유도형일양화담합매mRNA재뇌결혈재관주뇌손상중구유감경혈뇌병장적파배,보호혈관내피화뇌조직적작용.목적:관찰전침수구、내관、족삼리대뇌결혈재관주대서해마유도형일양화담합매mRNA표체적영향.설계:수궤대조실험.단위:상해중의약대학、상해시침구경락연구소급복단대학화산의원.방법:실험우2003-12/2004-12재상해중의약대학실험동물중심、상해시침구경락연구소침구면역학실험실화복단대학화산의원완성.40지SD대서수궤분위4조:정상조、가수술조、모형조화전침치료조,매조10지.용쌍신쌍협법복제역졸중형신혈관성고혈압모형,재차기출상,운용전선법제비대서국조성뇌결혈재관주모형;가수술조제불삽입전선외,기여보취동모형조;전침치료조취수구(진렬비첨하1 mm정중처,향상사자1 mm)、쌍측내관(전지내측,리완관절약3 mm처적척뇨골봉간,직자1 mm)、쌍측족삼리(슬관절하측,재비골소두하약5 mm처,직자7 mm)수구혈여우이근부피부、내관여족삼리접G6805전침치료의,련속파,빈솔120차/min,강도1 mA,류침30 min.결혈후즉시치료1차,재관주후매12 h치료1차.소유동물우재관주후24h단두처사,취출뇌조직,분리해마,응용형광정량역전록취합매련반응기술관찰전침대실험성뇌결혈재관주대서해마유도형일양화담합매mRNA표체적영향.주요관찰지표:대서해마유도형일양화담합매mRNA적표체.결과:40지SD대서균진입결과분석.대서해마유도형일양화담합매mRNA적표체:[1]모형조명현고우전침치료조{[(4.85±1.29)×1 000,(3.19±1.38)×1 000]고패,(t=2.77,P＜0.05)};[2]모형조명현고우가수술조{[(4.85±1.29)×1 000,(4.93±2.17)×10]고패,(t=97.38,P＜0.01)};[3]모형조현저고우정상조{[(4.85±1.29)×1 000,(3.13±1.68)×10]고패,(t=11.81,P＜0.01)}.결론:전침가이현저억제뇌결혈재관주후대서해마유도형일양화담합매mRNA표체,종이감소일양화담적산생,유조우감경뇌결혈재관주손상.
BACKGROUND: Researches indicate that inducible nitric oxide synthase(iNOS) plays an important role in relieving destruction of blood brain barrier and protecting vascular endothelium and brain tissue in cerebral ischemia-reperfusion injury.OBJECTIVE: To observe the effect of Shuigou (DU26), Neiguan (PC6)and Zusanli (ST36) on the expression of inducible nitric oxide synthase messenger ribonucleic acid (iNOS mRNA) in hippocampus of the rats with cerebral ischemia-reperfusion.DESIGN: Randomized controlled study.SETTING: Shanghai University of Traditional Chinese Medicine, Shanghai Institute of Acupuncture and Meridian and Huashan Hospital Affiliated to Fudan University.METHODS: The experiment was completed at the Experimental Animal Center in Shanghai University of Traditional Chinese Medicine, Threegrade Acupuncture Immunological Laboratory of Shanghai Research Institute of Acupuncture and Meridian and Huashan Hospital Affiliated to Fudan University from December 2003 to December 2004. Totally 40 SD rats were randomly divided as normal group, sham operation group, model group and electroacupuncture group with 10 in each group. The hypertension model rats were induced with bi-kidney-bi-clip method, then the focal cerebral ischemia-reperfusion rat model was induced by reversible occlusion for a middle cerebral artery with a thread on the stroke prone renovascular hypertensive model rat. While the rats in sham operation group were operated without thread insertion. Shuigou (Du 26), bilateral Neiguan(PC 6) and bilateral Housanli (Zusanli, ST 36) were located according to"Atlas of Experimental Animal Acupoints" made by Hua's. Shuigou (Du26): 1 mm below nasal apex right on the Labium leporinum, be punctured 1 mm obliquely upward; Neiguan (PC 6): at the lateral palm of the forearm3 mm above wrist joint, between ulna and radius, be punctured 1 mm perpendicularly; Housanli (Zusanli, ST 36): under knee joint, 5 mm below the capitulum of fibula, be punctured 7 mm perpendicularly. The skin of Shuigou (Du 26) and the root of right ear, Neiguan (PC 6) and Housanli (Zusanli, ST 36) were connected with electroacupuncture Therapeutic Instrument G6805 with the parameters of continuous wave, 120 times/minute in frequency, 1 mA in intensity for 30 minutes. The treatment was applied once immediately after ischemia operation and again 12 hours after reperfusion. Being killed on 24 hours after reperfusion, the hippocampus of the rats were rapidly picked out. The effect of electroacupuncture on the expression of iNOS mRNA in hippocampus of the rats with cerebral ischemia-reperfusion was detected with fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR).MAIN OUTCOME MEASURES: The expression of iNOS mRNA in hippocampus.RESULTS: All 40 SD rats entered the final analysis. Expression of iNOS mRNA in hippocampus: [1] That in model group was significant higher than that in electroacupuncture group {[(4.85±1.29) ×1 000, (3.19±1.38) ×1 000] copy, (t=2.77, P ＜ 0.05)}; [2] That in model group was significant higher than that in sham operation group {[(4.85±1.29) ×1 000, (4.93±2.17)×10] copy, (t=97.38, P ＜ 0.01)}; [3] That in model group was significant higher than that in normal group {[(4.85±1.29) ×1 000, (3.13±1.68) ×10]copy, (t=11.81, P ＜ 0.01)}.CONCLUSION: Electroacupuncture may significantly inhibit the expression of iNOS mRNA in hippocampus and reduce the synthesis of NO to relieve the cerebral ischemia-reperfusion injury.
