西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2006年
1期
4-10,41
,共8页
丘脑中央下核%阿片受体%伤害感受性调制%福尔马林试验%大鼠
丘腦中央下覈%阿片受體%傷害感受性調製%福爾馬林試驗%大鼠
구뇌중앙하핵%아편수체%상해감수성조제%복이마림시험%대서
nucleus submedius%opioid receptor%nociceptive modulation%formalin test%rat
目的研究μ-和δ-阿片受体在介导丘脑中央下核(Sm)内阿片诱发的抗伤害感受效应中的作用.方法用自动检测系统记录大鼠爪底皮下注射福尔马林诱发的伤害性行为,观察Sm内微量注射吗啡和选择性μ-和+δ-阿片受体拮抗剂对伤害性行为的效应.结果吗啡(31 mmol/L,0.5 μL)明显抑制福尔马林诱发的伤害性行为,这种效应可被μ-阿片受体拮抗剂β-funaltrexamine(β-FNA,0.4 mmol/L,0.5 μL)和naloxonazine(0.8 mmol/L,0.5 μL)阻断,部分地被δ-受体拮抗剂naltrindole(0.4 mmol/L,0.5 μL)阻断.结论Sm内注射吗啡诱发的抗伤害感受效应主要由μ-阿片受体介导,部分地由δ受体介导.
目的研究μ-和δ-阿片受體在介導丘腦中央下覈(Sm)內阿片誘髮的抗傷害感受效應中的作用.方法用自動檢測繫統記錄大鼠爪底皮下註射福爾馬林誘髮的傷害性行為,觀察Sm內微量註射嗎啡和選擇性μ-和+δ-阿片受體拮抗劑對傷害性行為的效應.結果嗎啡(31 mmol/L,0.5 μL)明顯抑製福爾馬林誘髮的傷害性行為,這種效應可被μ-阿片受體拮抗劑β-funaltrexamine(β-FNA,0.4 mmol/L,0.5 μL)和naloxonazine(0.8 mmol/L,0.5 μL)阻斷,部分地被δ-受體拮抗劑naltrindole(0.4 mmol/L,0.5 μL)阻斷.結論Sm內註射嗎啡誘髮的抗傷害感受效應主要由μ-阿片受體介導,部分地由δ受體介導.
목적연구μ-화δ-아편수체재개도구뇌중앙하핵(Sm)내아편유발적항상해감수효응중적작용.방법용자동검측계통기록대서조저피하주사복이마림유발적상해성행위,관찰Sm내미량주사마배화선택성μ-화+δ-아편수체길항제대상해성행위적효응.결과마배(31 mmol/L,0.5 μL)명현억제복이마림유발적상해성행위,저충효응가피μ-아편수체길항제β-funaltrexamine(β-FNA,0.4 mmol/L,0.5 μL)화naloxonazine(0.8 mmol/L,0.5 μL)조단,부분지피δ-수체길항제naltrindole(0.4 mmol/L,0.5 μL)조단.결론Sm내주사마배유발적항상해감수효응주요유μ-아편수체개도,부분지유δ수체개도.
Objective To investigate whether the μ- and δ-opioid receptors were involved in mediating the a ntinociceptive effect of opioid injection into the nucleus submedius (Sm). Methods Nociceptive behavior produced by subcutaneous injection of formalin (65 mmol/L, 50 μL) into the hind paw of the rat was assessed quantitatively using an automated movement detection system. The effects of morphine and selectiveμ- and δ-opioid receptor antagonists microinjected unilaterally into the Sm were determined in the awake rats. Results Morphine (31 mmol/L, 0. 5 μL) depressed the nociceptive behavior elicited by formalin, and this effect was antagonized completely by the selective μ-receptor antagonist β-funaltrexamine (β-FNA, 0. 4 mmol/L, 0. 5 μL) and naloxonazine (0.8 mmol/L, 0.5 μL), and partly by the δ-receptor antagonist naltrindole (0.4 mmol/L, 0.5μL).Administration of morphine into thalamic regions more than 0. 5 mm dorsal to the Sm had no effect on the nociceptive behavior. Conclusion Antinociceptive effects produced by opioid acting on Sm neurons are mediated mainly by μ-opioid receptors, and partly by δ-receptors.
