国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2009年
24期
1473-1477
,共5页
满晓燕%童朝晖%黄克武%庞宝森%詹庆元%张隽
滿曉燕%童朝暉%黃剋武%龐寶森%詹慶元%張雋
만효연%동조휘%황극무%방보삼%첨경원%장준
可溶性髓细胞表达触发受体1%重症肺炎%间质性肺炎%支气管肺泡灌洗液%促炎症细胞因子
可溶性髓細胞錶達觸髮受體1%重癥肺炎%間質性肺炎%支氣管肺泡灌洗液%促炎癥細胞因子
가용성수세포표체촉발수체1%중증폐염%간질성폐염%지기관폐포관세액%촉염증세포인자
Soluble triggering receptor expressed on myeloid cells-1%Severe pneumonia%Interstitial lung diseases%Bronchoalveolar lavage fluid%Proinflammatory cytokines
目的 探讨可溶性髓细胞表达触发受体1(soluble triggering receptor expressed on myeloid cells-1,sTREM-1)在感染性肺炎诊断中的价值及意义.方法 应用固相酶联免疫吸附试验法检测42例感染性肺炎(简称肺炎)患者(重症肺炎27例,非重症肺炎患者15例)、19例非感染性间质性肺炎(简称间质性肺炎)患者和9名健康人(正常对照组)支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血浆中sTREM-1、肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、IL-6的水平.结果①重症肺炎组及非重症肺炎组BALF中sTREM-1水平均高于正常对照组,(50.27±18.73)ng/L,(42.83±15.49)ng/Lvs(20.58±6.92)ng/L(P值均<0.01);间质性肺炎组BALF中sTREM-1水平[(22.77±5.90)ng/L]与正常对照组差异无统计学意义.②重症肺炎组血浆sTREM-1水平明显高于非重症肺炎组.(76.36±36.06)ng/L vs(19.46±7.73)ng/L(P<0.01);非重症肺炎组血浆sTREM-1水平高于间质性肺炎组及正常对照组,(19.46±7.73)ng/L vs(3.42±1.89)ng/L,(3.53±1.53)ng/L(P值均<0.01).③BALF及血浆中TNF-α、IL-6水平均表现为重症肺炎组高于非重症肺炎组及间质性肺炎组(P值均<0.01);非重症肺炎组及间质性肺炎组均高于正常对照组(P值均<0.05).BALF中IL-1β水平在重症肺炎组、非重症肺炎组及问质性肺炎组均高于正常对照组(P<0.05或P<0.01).结论 sTREM-1可以作为感染性肺炎有意义的辅助诊断指标,并有可能协助判断感染的严重程度.
目的 探討可溶性髓細胞錶達觸髮受體1(soluble triggering receptor expressed on myeloid cells-1,sTREM-1)在感染性肺炎診斷中的價值及意義.方法 應用固相酶聯免疫吸附試驗法檢測42例感染性肺炎(簡稱肺炎)患者(重癥肺炎27例,非重癥肺炎患者15例)、19例非感染性間質性肺炎(簡稱間質性肺炎)患者和9名健康人(正常對照組)支氣管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血漿中sTREM-1、腫瘤壞死因子α(TNF-α)、白介素1β(IL-1β)、IL-6的水平.結果①重癥肺炎組及非重癥肺炎組BALF中sTREM-1水平均高于正常對照組,(50.27±18.73)ng/L,(42.83±15.49)ng/Lvs(20.58±6.92)ng/L(P值均<0.01);間質性肺炎組BALF中sTREM-1水平[(22.77±5.90)ng/L]與正常對照組差異無統計學意義.②重癥肺炎組血漿sTREM-1水平明顯高于非重癥肺炎組.(76.36±36.06)ng/L vs(19.46±7.73)ng/L(P<0.01);非重癥肺炎組血漿sTREM-1水平高于間質性肺炎組及正常對照組,(19.46±7.73)ng/L vs(3.42±1.89)ng/L,(3.53±1.53)ng/L(P值均<0.01).③BALF及血漿中TNF-α、IL-6水平均錶現為重癥肺炎組高于非重癥肺炎組及間質性肺炎組(P值均<0.01);非重癥肺炎組及間質性肺炎組均高于正常對照組(P值均<0.05).BALF中IL-1β水平在重癥肺炎組、非重癥肺炎組及問質性肺炎組均高于正常對照組(P<0.05或P<0.01).結論 sTREM-1可以作為感染性肺炎有意義的輔助診斷指標,併有可能協助判斷感染的嚴重程度.
목적 탐토가용성수세포표체촉발수체1(soluble triggering receptor expressed on myeloid cells-1,sTREM-1)재감염성폐염진단중적개치급의의.방법 응용고상매련면역흡부시험법검측42례감염성폐염(간칭폐염)환자(중증폐염27례,비중증폐염환자15례)、19례비감염성간질성폐염(간칭간질성폐염)환자화9명건강인(정상대조조)지기관폐포관세액(bronchoalveolar lavage fluid,BALF)급혈장중sTREM-1、종류배사인자α(TNF-α)、백개소1β(IL-1β)、IL-6적수평.결과①중증폐염조급비중증폐염조BALF중sTREM-1수평균고우정상대조조,(50.27±18.73)ng/L,(42.83±15.49)ng/Lvs(20.58±6.92)ng/L(P치균<0.01);간질성폐염조BALF중sTREM-1수평[(22.77±5.90)ng/L]여정상대조조차이무통계학의의.②중증폐염조혈장sTREM-1수평명현고우비중증폐염조.(76.36±36.06)ng/L vs(19.46±7.73)ng/L(P<0.01);비중증폐염조혈장sTREM-1수평고우간질성폐염조급정상대조조,(19.46±7.73)ng/L vs(3.42±1.89)ng/L,(3.53±1.53)ng/L(P치균<0.01).③BALF급혈장중TNF-α、IL-6수평균표현위중증폐염조고우비중증폐염조급간질성폐염조(P치균<0.01);비중증폐염조급간질성폐염조균고우정상대조조(P치균<0.05).BALF중IL-1β수평재중증폐염조、비중증폐염조급문질성폐염조균고우정상대조조(P<0.05혹P<0.01).결론 sTREM-1가이작위감염성폐염유의의적보조진단지표,병유가능협조판단감염적엄중정도.
Objective To investigate the role of soluble triggering receptor expressed on myeloid cells-l(sTREM-1)in the diagnosis of infectious pneumonia. Methods Forty-two patients with infectious pneumonia(including 27 severe pneumonia patients and 15 non-severe pneumonia patients)and 19 patients with noninfectious interstitial lung diseases(ILD) were enrolled in the study. Nine healthy volunteers were recruited as controls, sTREM-1, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 in.bronchoalveolar lavage fluid (BALF)and plasma were measured by a solid-phase enzyme-linked immunosorbent assay. Results ① sTREM-1 levels in BALF of severe pneumonia group and non-severe pneumonia group were higher than that in normal control group, (50. 27±18. 73)ng/L,(42. 83± 15.49)ng/Lvs (20. 58±6.92) ng/L(all P <0. 01). There was no statistical significance between ILD group[(22.77±5.90) ng/L]and normal control group. ② sTREM-1 level in plasma of severe pneumonia group was higher than that in non-severe pneumonia group, ( 76.36 ± 36.06 ) ng/L vs ( 19.46 ± 7, 73 )ng/ L ( P < 0.01 ).sTREM-1 level in plasma of non-severe pneumonia group was higher than that in ILD group and normal control group,(19.46 ± 7.73) ng/L vs (3. 42 ± 1.89) ng/L, (3.53 ± 1.53) ng/L( P <0.01), ③TNF-αand IL-6 in BALF and plasma of severe pneumonia group were higher than those in non-severe pneumonia group and ILD group(all P <0.01). TNF-α and IL-6 in BALF and plasma of non-severe pneumonia group and ILD group were higher than those in normal control group(all P <0.05). IL-1β levels in BALF of severe pneumonia group, non-severe pneumonia group and ILD group were higher than that in normal control group( P < 0.05 or P < 0.01 ). Conclusions sTREM-1 ean be a beneficial biomarker in the diagnosis of infectious pneumonia.and the assay of sTREM-1 in BALF and plasma can help evaluate the severity of infection.
