CAJ | 학술논문

目的探讨肝移植术后ImmuKnow细胞免疫功能测定值与白细胞分类计数和T淋巴细胞亚群计数的相关性,为临床提供一种价格低廉快速判断肝移植受者细胞免疫功能的方法.方法选择49例行经典原位肝移植术受者术后2周至2个月内在无糖皮质激素应用情况下的外周血样本.分析ImmuKnow测定值与白细胞分类计数和T淋巴细胞亚群计数的相关性.并随机选择5例无激素免疫抑制的移植受者于术后2、3、4、6、8周分别重复检测上述指标,进一步验证其相关性.结果白细胞总数与ImmuKnow ATP值相关性最高,相关系数为0.821;中性粒细胞计数与ImmuKnow ATP值相关性次之,相关系数为0.787;单核细胞计数相关系数虽然有统计学意义,但相关系数低于0.5.淋巴细胞计数和淋巴细胞亚群计数与ImmuKnow ATP值的相关性无统计学意义.5例无激素免疫抑制受者术后重复检测ImmuKnow ATP值的变化与细胞总数的变化呈正相关,相关系数均>0.5.结论肝移植术后早期白细胞计数与CD4+T细胞ImmuKnow ATP值具有一定的正相关性,白细胞计数的变化,可以在一定程度上反映ImmuKnow ATP值的变化.
목적 탐토간이식술후ImmuKnow세포면역공능측정치여백세포분류계수화T림파세포아군계수적상관성,위림상제공일충개격저렴쾌속판단간이식수자세포면역공능적방법.방법 선택49례행경전원위간이식술수자술후2주지2개월내재무당피질격소응용정황하적외주혈양본.분석ImmuKnow측정치여백세포분류계수화T림파세포아군계수적상관성.병수궤선택5례무격소면역억제적이식수자우술후2、3、4、6、8주분별중복검측상술지표,진일보험증기상관성.결과 백세포총수여ImmuKnow ATP치상관성최고,상관계수위0.821;중성립세포계수여ImmuKnow ATP치상관성차지,상관계수위0.787;단핵세포계수상관계수수연유통계학의의,단상관계수저우0.5.림파세포계수화림파세포아군계수여ImmuKnow ATP치적상관성무통계학의의.5례무격소면역억제수자술후중복검측ImmuKnow ATP치적변화여세포총수적변화정정상관,상관계수균>0.5.결론 간이식술후조기백세포계수여CD4+T세포ImmuKnow ATP치구유일정적정상관성,백세포계수적변화,가이재일정정도상반영ImmuKnow ATP치적변화.
Objective To explore the relationship between peripheral differential blood count and ATP value in Cell CD4 + T tested by ImmuKnow method in liver transplants. Methods In this study 49recipients after classic orthotopic liver transplantation (OLT) were enrolled. In a period from two weeks to two months after transplantation when all were free of glucocorticoid. Blood were sent for WBC differential samples count and ATP value in Cell-CD4 + T tested by ImmuKnow method via SPSS17. 0 software. Five more samples were selected randomly for duplicated testing of the indices in Week2, 3, 4,6 and 8 after the transplanting operation to further verify the relativity. Results White blood cell count has the highest relativity with ImmuKnow ATP value at 0. 821. The 5 recipients were repeatedly tested for ImmuKnow ATP values that were found positively correlated to cell count with a coefficient of over 0. 5. Conclusions The peripheral leukocyte count in early stage after liver transplantation is in positive correlation with ATP value in Cell CD4 + T, and the changes of numeration of leukocyte reflect changes of ATP value.