中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2009年
2期
110-113
,共4页
黎润光%邵景范%魏明发%洪振亚%杨勇%杨小进%周学峰%陈超%冯杰雄%袁继炎
黎潤光%邵景範%魏明髮%洪振亞%楊勇%楊小進%週學峰%陳超%馮傑雄%袁繼炎
려윤광%소경범%위명발%홍진아%양용%양소진%주학봉%진초%풍걸웅%원계염
牵张应力%间质干细胞%细胞分化
牽張應力%間質榦細胞%細胞分化
견장응력%간질간세포%세포분화
Mechanical strain%Mesenchymal stem ceils:Cell differentiation
目的 探讨周期性机械应力对小儿骨髓问充质干细胞(MSCs)分化方向的影响.方法 利用自行建立的机械牵张应力装置,对体外MSCs加载力学信号,并复合成脂肪诱导剂,选用化学染色、RT-PRC、免疫组化、Western Blot的方法检测MSCs干预后成骨指标(OC、BMP-2、I型胶原)和成脂肪分化的指标(PPART-2、脂滴).结果 MSCS受力后,细胞OC mRNA表达较对照组明显增高,而且与干预时间相关;干预7 d后,细胞内BMP-2、I型胶原蛋白表达较对照组明显增强;MSCs在成脂肪诱导过程中,接受应力后细胞脂滴的出现和PPARy-2 mRNA的表达均明显下降.结论 适当的应力促进MSCs向成骨方向分化,抑制其向脂肪方向分化.
目的 探討週期性機械應力對小兒骨髓問充質榦細胞(MSCs)分化方嚮的影響.方法 利用自行建立的機械牽張應力裝置,對體外MSCs加載力學信號,併複閤成脂肪誘導劑,選用化學染色、RT-PRC、免疫組化、Western Blot的方法檢測MSCs榦預後成骨指標(OC、BMP-2、I型膠原)和成脂肪分化的指標(PPART-2、脂滴).結果 MSCS受力後,細胞OC mRNA錶達較對照組明顯增高,而且與榦預時間相關;榦預7 d後,細胞內BMP-2、I型膠原蛋白錶達較對照組明顯增彊;MSCs在成脂肪誘導過程中,接受應力後細胞脂滴的齣現和PPARy-2 mRNA的錶達均明顯下降.結論 適噹的應力促進MSCs嚮成骨方嚮分化,抑製其嚮脂肪方嚮分化.
목적 탐토주기성궤계응력대소인골수문충질간세포(MSCs)분화방향적영향.방법 이용자행건립적궤계견장응력장치,대체외MSCs가재역학신호,병복합성지방유도제,선용화학염색、RT-PRC、면역조화、Western Blot적방법검측MSCs간예후성골지표(OC、BMP-2、I형효원)화성지방분화적지표(PPART-2、지적).결과 MSCS수력후,세포OC mRNA표체교대조조명현증고,이차여간예시간상관;간예7 d후,세포내BMP-2、I형효원단백표체교대조조명현증강;MSCs재성지방유도과정중,접수응력후세포지적적출현화PPARy-2 mRNA적표체균명현하강.결론 괄당적응력촉진MSCs향성골방향분화,억제기향지방방향분화.
Objective To study the correlation between mechanical strain and the differentiation of mesenchymal stem cells(MSCs)of children's bone marrow tO osteoblasts and adipocytes in vitro.Methods The MSCS were isolated and primary cultured.After 3rd-6th passage of MSCs,mechanical stretch,and(or)condition medium was perform on MSCs.Cytochemical staining,RT-PCR,immunocytochemical staining and Western Blot were applied to measure the markers of osteoblasts(OC,BMP-2,I-col)and the markers of adipocytes(lipid droplets,PPARy-2).Results After mechanical stretch treatment,the level of intracellular OC mRNA was higher than that of the control group.which showed the correlation between the intracellular OC mRNA and the stimulating period.After stimulating the MSCS for 7days,the expression of BMP-2 and I-col was significantly higher than that of the control group.In the process of induction of MSCS to adipocytes,the mechanical stretched cells had less intracellular lipid droplet and lower expression of PPART-2 mRNA.Conclusions These data suggest proper mechanical strain could improve the effect on the differentiation of MSCs to osteoblasts.while inhibit the effect on the differentiation of MSCS to adipocytes.
