CAJ | 학술논문

目的建立稳定、理想的大鼠30%体积肝移植模型.方法二袖套法和体内切肝法建立非动脉化30%体积肝移植模型,对100只SD大鼠施行50例30%体积肝移植(10例前期操作,不列入统计),对手术操作技巧和细节、术后存活及原因等进行探讨.结果手术成功率100%(40/40),术后1周、2周存活率均40%(16/40),死亡原因主要为肝功能失代偿、胆管梗阻或胆漏、肝上下腔静脉血栓形成、肝叶坏死和腹腔感染等.结论 30%体积肝移植模型改进后供肝残面出血率低,机械损伤小,供肝再灌注色红均匀,存活率好,可作为小体积肝移植有关实验研究的动物模型.
목적 건립은정、이상적대서30%체적간이식모형.방법 이수투법화체내절간법건립비동맥화30%체적간이식모형,대100지SD대서시행50례30%체적간이식(10례전기조작,불렬입통계),대수술조작기교화세절、술후존활급원인등진행탐토.결과 수술성공솔100%(40/40),술후1주、2주존활솔균40%(16/40),사망원인주요위간공능실대상、담관경조혹담루、간상하강정맥혈전형성、간협배사화복강감염등.결론 30%체적간이식모형개진후공간잔면출혈솔저,궤계손상소,공간재관주색홍균균,존활솔호,가작위소체적간이식유관실험연구적동물모형.
Objective To establish a reliable and ideal model of 30% size graft liver transplantation in rats. Methods The model of 30% size graft liver transplantation in vivo was established by using male SD rats according to Kamada's two-cuff method. The operative techniques and ditails, and survival rate were explored and analyzed. Results The rate of successful surgery was 100% (40/40). The one- and two-week survival rate both were 40% (16/40), and the causes of deaths included liver function decompensation, bile duct stenosis or bile leakage, thrombosis of super inferior vena cava, liver necrosis and celiac infection.Conclusion Improved model of 30% small-for-size liver transplantation had a low hemorrhage rate of residual liver surface, reduced mechanical damage in small-for-size graft, high success rate and survival rate, and was an ideal animal model on the study of experiments related to the small-for-size liver transplantation.