CAJ | 학술논문

目的建立癫痫持续状态后颞叶癫痫模型,观察大鼠自发性反复发作(SRS)。方法持续电刺激大鼠杏仁核20 min(单相方波,强度400μA,波宽1 ms,串长100 ms,串隔0.5s,频率60Hz)引发癫痫持续状态(SE),随后进行3h的SE观察,包括大鼠行为表现和脑电变化。SE状态引出后,进行3个月的视频监测,观察大鼠自发性发作的发作情况并进行分组。结果经过为期3个月的视频观察,出现SRS14只(67％);未出现者7只(33％)。共记录到3～5级发作181次。其中3级发作10次(5.53％),4级发作77次(42.54％),5级发作94次(51.93％)。发作在30次以上者3只;20 ～ 30次者1只;10 ～20次者2只,5～10次者2只;5次以下者6只。约73％的SRS出现在白天(07:00～19:00),74％的继发性全身发作(4、5级发作)出现于白天。结论成功制备了癫痫持续状态后颞叶癫痫动物模型。
목적 건립전간지속상태후섭협전간모형,관찰대서자발성반복발작(SRS)。방법 지속전자격대서행인핵20 min(단상방파,강도400μA,파관1 ms,천장100 ms,천격0.5s,빈솔60Hz)인발전간지속상태(SE),수후진행3h적SE관찰,포괄대서행위표현화뇌전변화。SE상태인출후,진행3개월적시빈감측,관찰대서자발성발작적발작정황병진행분조。결과 경과위기3개월적시빈관찰,출현SRS14지(67％);미출현자7지(33％)。공기록도3～5급발작181차。기중3급발작10차(5.53％),4급발작77차(42.54％),5급발작94차(51.93％)。발작재30차이상자3지;20 ～ 30차자1지;10 ～20차자2지,5～10차자2지;5차이하자6지。약73％적SRS출현재백천(07:00～19:00),74％적계발성전신발작(4、5급발작)출현우백천。결론 성공제비료전간지속상태후섭협전간동물모형。
Objective To establish a reliable post-status epilepticus model of temporal lobe epilepsy in rats that mimics human temporal lobe epilepsy, and observe the occurrence of spontaneous recurrent seizures (SRS). Methods Status epilepticus which was monitored for 3 h was induced by a 20-min stimulation of the amygdala ( 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0. 5 s). Stimulated rats ( n =21 ) were monitored with a video recording system for the 3-months follow-up period to record the appearance of SRS. Results SRS (total number 181 ) were detected in 14 of the 21 animals (67％) after a latency period of 8 to 73 days (mean 43 days), including 10 times of stage 3 seizure (5.53％), 77 times of stage 4 seizure (42. 54％ ), and 94 times of stage 5 seizure (51.93％). Of all the 14 rats, the seizure number beyond 30 was observed in 3 animals, from 20 to 30 in one animal, from 10 to 20 in 2 animals, between 5 and 10 in 2 animals, and less than 5 in 6 animals. Seventy-three percent of SRS appeared during daytime (07: 00-19: 00) and 74 percent of secondarily generalized seizures occurred during daytime. Conclusion This study has been successfully designed to develop a post-status epilepticus animal model induced by electrical stimulation of the amygdale.